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Rabbit Recombinant Multiclonal CCR9 antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry - Anti-CCR9 antibody [RM2031] (AB315484), expandable thumbnail
  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (AB315484), expandable thumbnail
  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (AB315484), expandable thumbnail
  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (AB315484), expandable thumbnail
  • Western blot - Anti-CCR9 antibody [RM2031] (AB315484), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow CytICC/IFIPIHC-P
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Expected
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

1 product for Alternative Version

Target data

Function

Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

Alternative names

CDw199, GPR28, CCR9, C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6

Recommended products

Rabbit Recombinant Multiclonal CCR9 antibody. Suitable for WB, Flow Cyt, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM2031
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CCR9 also known as CD199 is a chemokine receptor known for its role in guiding cell traffic. It is a G-protein-coupled receptor with a mass of approximately 43 kDa. CCR9 is mainly expressed on the surface of thymocytes T lymphocytes and some subsets of dendritic cells. Its presence is especially noted in the small intestine correlated with its role in the migration and function of immune cells in the gut. This receptor is important for the homing of these cells to the intestinal mucosa engaging specifically with its ligand CCL25.

Biological function summary

CCR9 plays a significant role in the immune system particularly in the gut. It is part of chemokine receptor-ligand complexes that are integral to leukocyte trafficking. By interacting with CCL25 CCR9 facilitates the directed movement of immune cells across intestinal tissue impacting the immune surveillance and response within this environment. The receptor's expression pattern also suggests involvement in thymocyte development supporting the immune system's ability to generate a diverse repertoire of T cells.

Pathways

CCR9 is an important component of the chemokine signaling pathway. It is intricately connected to G-protein signaling events triggering downstream effects that drive cell migration and adhesion. This pathway is important for the function and maintenance of mucosal immunity linking CCR9 with CCR7 and its role in guiding T cell migration to specific tissue sites. These pathways collectively support immune functionality and homeostasis underlining CCR9's significance in tissue-specific immunity.

Associated diseases and disorders

CCR9 has associations with inflammatory disorders like Crohn's disease and ulcerative colitis. Inflammatory bowel disease (IBD) is marked by aberrant immune cell movement and function areas where CCR9 is particularly influential. The receptor's interaction with CCL25 is pivotal in these pathological conditions often being a therapeutic target to modulate immune cell trafficking in disease management. Additionally the altered expression of CCR9 may contribute to the progression of specific T-cell leukemias further emphasizing its clinical relevance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484)

    Flow cytometric analysis of HepG2 (human hepatocellular carcinoma epithelial cell, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab315484 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: HepG2. Gated on viable cells.

  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with ab315484 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells. Cells are co-stained with CD4 conjugated to Alexa Fluor®647.

  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR9 with ab315484 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells. Cells are co-stained with CD56 conjugated to BV421.

  • Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Flow Cytometry - Anti-CCR9 antibody [RM2031] (ab315484)

    Flow cytometric analysis of Mouse thymocytes cells labelling CCR9 with ab315484 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Gated on viable double positive CD4+ CD8a+ cells.

  • Western blot - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Western blot - Anti-CCR9 antibody [RM2031] (ab315484)

    Low expression: HEK-293 (PMID: 24870448).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CCR9 antibody [RM2031] (ab315484) at 1/1000 dilution

    Lane 1: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: CTLL-2 (mouse T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 6: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 42 kDa, 36 kDa

    Exposure time: 136s

  • Western blot - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Western blot - Anti-CCR9 antibody [RM2031] (ab315484)

    Low expression: skeletal muscle.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CCR9 antibody [RM2031] (ab315484) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human lymphma tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 42 kDa, 36 kDa

    Exposure time: 70s

  • Western blot - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Western blot - Anti-CCR9 antibody [RM2031] (ab315484)

    Low expression: HepG2 (PMID: 24481516).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CCR9 antibody [RM2031] (ab315484) at 1/1000 dilution

    Lane 1: MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 42 kDa, 36 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [RM2031] (ab315484)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling CCR9 with ab315484 at 1/50 (9.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in EL4 cell line.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [RM2031] (ab315484), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [RM2031] (ab315484)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling CCR9 with ab315484 at 1/50 (9.84 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing membranous staining in MOLT-4 cells.

    Low expression: HepG2 (PMID: 24481516).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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