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AB109184

Anti-CCT2 antibody [EPR4083]

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(3 Publications)

Rabbit Recombinant Monoclonal CCT2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

99D8.1, CCTB, CCT2, T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

3 Images
Western blot - Anti-CCT2 antibody [EPR4083] (AB109184)
  • WB

Unknown

Western blot - Anti-CCT2 antibody [EPR4083] (AB109184)

All lanes:

Western blot - Anti-CCT2 antibody [EPR4083] (ab109184) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF-7 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 57 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4083] (AB109184)

Immunofluorescence staining of HeLa cells with purified ab109184 at a working dilution of 1/500 counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077) used at a dilution of 1/1000. The cells were fixed in 100% methanol. The negative control is shown in bottom right hand panel - for the negative control PBS was used instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4083] (AB109184)

ab109184 at 1/100 dilution staining CCT2 in Human kidney tissue by Immunohistochemistry

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4083

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCT2 also known as Chaperonin Containing TCP1 Subunit 2 is part of a group of proteins responsible for facilitating the correct folding of other proteins. It functions as a molecular chaperone and has a molecular weight of approximately 58 kDa. CCT2 is expressed in many tissues with high levels detected in testis and brain tissues. This protein is integral to the chaperonin CCT (or TRiC) complex contributing to essential cellular processes through its role in protein homeostasis.
Biological function summary

CCT2 assists in the folding of cytoskeletal proteins like actin and tubulin which are vital for maintaining the structure and function of cells. As part of the CCT complex which consists of eight different subunits CCT2 plays a significant role in ensuring the proper assembly of multi-protein complexes. The activity of CCT2 is important for the prevention of aggregate formation which otherwise leads to cellular stress.

Pathways

CCT2 significantly influences the protein folding and degradation pathways. It participates in the cellular pathways that govern the proteostasis network collaborating with other chaperones to oversee protein quality control. Related proteins interacting with CCT2 within these pathways include other CCT complex components like CCT3 and CCT4 all contributing to maintaining cellular homeostasis and protecting cells from proteotoxic damage.

CCT2 is connected to cancer and neurodegenerative diseases. Altered expression of CCT2 is linked to the progression of tumor growth interacting with proteins involved in cell cycle regulation and signaling such as p53. Additionally deficits in CCT2 function can exacerbate protein misfolding disorders like Alzheimer's disease where proteins like tau become aggregated contributing to the pathology. Efforts to understand CCT2's role in these conditions highlight the importance of protein homeostasis in maintaining health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
See full target information CCT2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled

Frontiers in oncology 11:663877 PubMed33996588

2021

Chaperonin-Containing TCP1 Complex (CCT) Promotes Breast Cancer Growth Through Correlations With Key Cell Cycle Regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Heba Ghozlan,Adrian Showalter,Eunkyung Lee,Xiang Zhu,Annette R Khaled

Scientific reports 10:798 PubMed31964905

2020

Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E Showalter,Ana C Martini,Daniel Nierenberg,Kristen Hosang,Naima Ahmed Fahmi,Priya Gopalan,Amr S Khaled,Wei Zhang,Annette R Khaled
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB92746

Anti-CCT2 antibody [EPR4084]

primary-antibodies

cct2-antibody-epr4084-ab92746

5

(2 reviews)

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