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Rabbit Recombinant Monoclonal CCT2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 11 publications.


Images

Western blot - Anti-CCT2 antibody [EPR4084] (AB92746), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (AB92746), expandable thumbnail
  • Western blot - Anti-CCT2 antibody [EPR4084] (AB92746), expandable thumbnail
  • Immunoprecipitation - Anti-CCT2 antibody [EPR4084] (AB92746), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] (AB92746), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/10000 - 1/50000

Notes

-

Species

Rat

Dilution info

1/10000 - 1/50000

Notes

-

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

For unpurified use at 1/100 - 1/250

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10 - 1/100

Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Alternative names

99D8.1, CCTB, CCT2, T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

Recommended products

Rabbit Recombinant Monoclonal CCT2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4084

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCT2 also known as Chaperonin Containing TCP1 Subunit 2 is part of a group of proteins responsible for facilitating the correct folding of other proteins. It functions as a molecular chaperone and has a molecular weight of approximately 58 kDa. CCT2 is expressed in many tissues with high levels detected in testis and brain tissues. This protein is integral to the chaperonin CCT (or TRiC) complex contributing to essential cellular processes through its role in protein homeostasis.

Biological function summary

CCT2 assists in the folding of cytoskeletal proteins like actin and tubulin which are vital for maintaining the structure and function of cells. As part of the CCT complex which consists of eight different subunits CCT2 plays a significant role in ensuring the proper assembly of multi-protein complexes. The activity of CCT2 is important for the prevention of aggregate formation which otherwise leads to cellular stress.

Pathways

CCT2 significantly influences the protein folding and degradation pathways. It participates in the cellular pathways that govern the proteostasis network collaborating with other chaperones to oversee protein quality control. Related proteins interacting with CCT2 within these pathways include other CCT complex components like CCT3 and CCT4 all contributing to maintaining cellular homeostasis and protecting cells from proteotoxic damage.

Associated diseases and disorders

CCT2 is connected to cancer and neurodegenerative diseases. Altered expression of CCT2 is linked to the progression of tumor growth interacting with proteins involved in cell cycle regulation and signaling such as p53. Additionally deficits in CCT2 function can exacerbate protein misfolding disorders like Alzheimer's disease where proteins like tau become aggregated contributing to the pathology. Efforts to understand CCT2's role in these conditions highlight the importance of protein homeostasis in maintaining health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Western blot - Anti-CCT2 antibody [EPR4084] (ab92746)

    Blocking/Diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-CCT2 antibody [EPR4084] (ab92746) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Human brain lysate at 15 µg

    Lane 3: Mouse brain lysate at 15 µg

    Lane 4: Rat brain lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa

    Observed band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling CCT2 with purified ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Western blot - Anti-CCT2 antibody [EPR4084] (ab92746)

    All lanes: Western blot - Anti-CCT2 antibody [EPR4084] (ab92746) at 1/10000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: Daudi cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunoprecipitation - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunoprecipitation - Anti-CCT2 antibody [EPR4084] (ab92746)

    Purified ab92746 at 1:30 dilution (2μg) immunoprecipitating CCT2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab92746 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab92746 in HeLa whole cell lysate.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 57 kDa

    All lanes: Immunoprecipitation - Anti-CCT2 antibody [EPR4084] (ab92746)

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] (ab92746)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling CCT2 with Purified ab92746 at 1:500 dilution (0.6 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CCT2 with purified ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling CCT2 with purified ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] (ab92746)

    ab92746, unpurified, at a 1/100 dilution, staining CCT2 in formalin fixed, paraffin embedded (1) Human colon tissue and (2) Human kidney tissue by Immunohistochemistry. Detection: DAB staining.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] (ab92746)

    ab92746, unpurified, at a 1/100 dilution, staining CCT2 in HeLa cells by Immunofluorescence.

  • Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] (ab92746)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells, labeling CCT2 with Purified ab92746 at 1/30 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] (ab92746), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] (ab92746)

    Overlay histogram showing HeLa cells stained with ab92746, unpurified, (red line), unpurified. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92746, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

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