Skip to main content

Rabbit Recombinant Monoclonal CCT2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (AB247598), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (AB247598), expandable thumbnail
  • Immunoprecipitation - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (AB247598), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (AB247598), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (AB247598), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Tested
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Version

Target data

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Alternative names

99D8.1, CCTB, CCT2, T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, Chaperonin containing T-complex polypeptide 1 subunit 2

Recommended products

Rabbit Recombinant Monoclonal CCT2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4084
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247598 is the carrier-free version of Anti-CCT2 antibody [EPR4084] ab92746.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CCT2 also known as Chaperonin Containing TCP1 Subunit 2 is part of a group of proteins responsible for facilitating the correct folding of other proteins. It functions as a molecular chaperone and has a molecular weight of approximately 58 kDa. CCT2 is expressed in many tissues with high levels detected in testis and brain tissues. This protein is integral to the chaperonin CCT (or TRiC) complex contributing to essential cellular processes through its role in protein homeostasis.

Biological function summary

CCT2 assists in the folding of cytoskeletal proteins like actin and tubulin which are vital for maintaining the structure and function of cells. As part of the CCT complex which consists of eight different subunits CCT2 plays a significant role in ensuring the proper assembly of multi-protein complexes. The activity of CCT2 is important for the prevention of aggregate formation which otherwise leads to cellular stress.

Pathways

CCT2 significantly influences the protein folding and degradation pathways. It participates in the cellular pathways that govern the proteostasis network collaborating with other chaperones to oversee protein quality control. Related proteins interacting with CCT2 within these pathways include other CCT complex components like CCT3 and CCT4 all contributing to maintaining cellular homeostasis and protecting cells from proteotoxic damage.

Associated diseases and disorders

CCT2 is connected to cancer and neurodegenerative diseases. Altered expression of CCT2 is linked to the progression of tumor growth interacting with proteins involved in cell cycle regulation and signaling such as p53. Additionally deficits in CCT2 function can exacerbate protein misfolding disorders like Alzheimer's disease where proteins like tau become aggregated contributing to the pathology. Efforts to understand CCT2's role in these conditions highlight the importance of protein homeostasis in maintaining health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Western blot - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Blocking/Diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

    All lanes: Western blot - Anti-CCT2 antibody [EPR4084] (Anti-CCT2 antibody [EPR4084] ab92746) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Human brain lysate at 15 µg

    Lane 3: Mouse brain lysate at 15 µg

    Lane 4: Rat brain lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 57 kDa

    Observed band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling CCT2 with purified Anti-CCT2 antibody [EPR4084] ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

  • Immunoprecipitation - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Immunoprecipitation - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Purified Anti-CCT2 antibody [EPR4084] ab92746 at 1:30 dilution (2μg) immunoprecipitating CCT2 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-CCT2 antibody [EPR4084] ab92746 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CCT2 antibody [EPR4084] ab92746 in HeLa whole cell lysate.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 57 kDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

    All lanes: Immunoprecipitation - Anti-CCT2 antibody [EPR4084] (Anti-CCT2 antibody [EPR4084] ab92746)

    Predicted band size: 57 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling CCT2 with Purified Anti-CCT2 antibody [EPR4084] ab92746 at 1:500 dilution (0.6 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CCT2 with purified Anti-CCT2 antibody [EPR4084] ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling CCT2 with purified Anti-CCT2 antibody [EPR4084] ab92746 at 1/24000 dilution (0.012 μg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

  • Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] - BSA and Azide free (ab247598)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CCT2 with Purified Anti-CCT2 antibody [EPR4084] ab92746 at 1/30 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS,BSA,glycerol,and sodium azide (Anti-CCT2 antibody [EPR4084] ab92746).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com