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AB176686

Anti-CCT3 antibody

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(1 Publication)

Rabbit Polyclonal CCT3 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 450 to C-terminus.

View Alternative Names

CCTG, TRIC5, CCT3, T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, Chaperonin containing T-complex polypeptide 1 subunit 3, hTRiC5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB176686)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB176686)

Paraffin embedded human lung carcinoma tissue stained for CCT3 using ab176686 at 1/200 dlution in immunohistochemical analysis. Counterstain : hematoxylin (blue).

Immunoprecipitation - Anti-CCT3 antibody (AB176686)
  • IP

Supplier Data

Immunoprecipitation - Anti-CCT3 antibody (AB176686)

Detection of Human CCT3 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176686 at 6 μg/mg lysate for IP and at 0.4 μg/ml for subsequent Western blot detection. Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CCT3 antibody (ab176686)

Predicted band size: 61 kDa

true

Exposure time: 3s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB176686)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB176686)

Paraffin embedded mouse teratoma tissue stained for CCT3 using ab176686 at 1/200 dlution in immunohistochemical analysis.

Western blot - Anti-CCT3 antibody (AB176686)
  • WB

Supplier Data

Western blot - Anti-CCT3 antibody (AB176686)

All lanes:

Western blot - Anti-CCT3 antibody (ab176686) at 0.04 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

HeLa cell lysate at 15 µg

Lane 3:

293T cell lysate at 50 µg

Lane 4:

Jurkat cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CCT3 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P49368

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab176686 was affinity purified using an epitope specific to CCT3 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCT3 also known as TCP-1-γ is a subunit of the chaperonin-containing TCP1 (CCT) complex also called the TRiC complex. This protein has a molecular mass of approximately 60 kDa and is expressed in many tissues but is especially abundant in the cytoplasm of eukaryotic cells. CCT3 plays an essential role in the folding of actin and tubulin which are critical for maintaining the cell's cytoskeleton structure. The mechanical function of CCT3 includes providing a folding chamber where newly synthesized polypeptides achieve their correct conformation preventing aggregation and ensuring functional protein production.
Biological function summary

CCT3 is a component of the TRiC/CCT complex which is important for protein quality control within the cell. This complex assists in folding a subset of proteins that are typically difficult to fold without help. CCT3 collaborates with other subunits in the complex to ensure proper assembly and function of structural proteins. Its role is particularly important in high turnover tissues or rapidly proliferating cells where efficient protein folding is essential to maintain cellular function and growth.

Pathways

CCT3 is involved in essential signaling cascades like the cytoskeleton regulation and protein homeostasis pathways. It has relationships with proteins such as actin and beta-tubulin which are critical parts of these pathways. The accurate folding facilitated by CCT3 influences the stability and function of these proteins impacting cell division motility and organization. The involvement in these pathways highlights CCT3’s contribution to maintaining cellular integrity and function.

Mutations or abnormal expression of CCT3 have been linked to neurodegenerative disorders and certain cancers. Dysregulation of this protein may contribute to amyotrophic lateral sclerosis (ALS) due to its role in protein homeostasis and cytoskeleton maintenance. In cancer overexpression of CCT3 has been observed in conditions such as hepatocellular carcinoma potentially promoting tumor growth through altered protein folding. The interaction of CCT3 with proteins like HSP70 during stress responses also connects it to processes that might protect against disease-related protein aggregation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
See full target information CCT3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:24357 PubMed34934095

2021

circRNA N6-methyladenosine methylation in preeclampsia and the potential role of N6-methyladenosine-modified circPAPPA2 in trophoblast invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Zhang,Hongling Yang,Yan Long,Yipeng Zhang,Ronggui Chen,Junzhu Shi,Jiying Chen
View all publications
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