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Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.

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Images

Western blot - Anti-CCT3 antibody (AB225878), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878), expandable thumbnail
  • Immunoprecipitation - Anti-CCT3 antibody (AB225878), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CCT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P49368

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Alternative names

Recommended products

Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CCT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link P49368
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCT3 also known as TCP-1-γ is a subunit of the chaperonin-containing TCP1 (CCT) complex also called the TRiC complex. This protein has a molecular mass of approximately 60 kDa and is expressed in many tissues but is especially abundant in the cytoplasm of eukaryotic cells. CCT3 plays an essential role in the folding of actin and tubulin which are critical for maintaining the cell's cytoskeleton structure. The mechanical function of CCT3 includes providing a folding chamber where newly synthesized polypeptides achieve their correct conformation preventing aggregation and ensuring functional protein production.

Biological function summary

CCT3 is a component of the TRiC/CCT complex which is important for protein quality control within the cell. This complex assists in folding a subset of proteins that are typically difficult to fold without help. CCT3 collaborates with other subunits in the complex to ensure proper assembly and function of structural proteins. Its role is particularly important in high turnover tissues or rapidly proliferating cells where efficient protein folding is essential to maintain cellular function and growth.

Pathways

CCT3 is involved in essential signaling cascades like the cytoskeleton regulation and protein homeostasis pathways. It has relationships with proteins such as actin and beta-tubulin which are critical parts of these pathways. The accurate folding facilitated by CCT3 influences the stability and function of these proteins impacting cell division motility and organization. The involvement in these pathways highlights CCT3’s contribution to maintaining cellular integrity and function.

Associated diseases and disorders

Mutations or abnormal expression of CCT3 have been linked to neurodegenerative disorders and certain cancers. Dysregulation of this protein may contribute to amyotrophic lateral sclerosis (ALS) due to its role in protein homeostasis and cytoskeleton maintenance. In cancer overexpression of CCT3 has been observed in conditions such as hepatocellular carcinoma potentially promoting tumor growth through altered protein folding. The interaction of CCT3 with proteins like HSP70 during stress responses also connects it to processes that might protect against disease-related protein aggregation.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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4 product images

  • Western blot - Anti-CCT3 antibody (ab225878), expandable thumbnail

    Western blot - Anti-CCT3 antibody (ab225878)

    All lanes: Western blot - Anti-CCT3 antibody (ab225878) at 0.04 µg/mL

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

    Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa

    Exposure time: 1s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878)

    Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

  • Immunoprecipitation - Anti-CCT3 antibody (ab225878), expandable thumbnail

    Immunoprecipitation - Anti-CCT3 antibody (ab225878)

    CCT3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225878 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225878 at 0.4 μg/ml.

    Lane 1: ab225878 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

    All lanes: Immunoprecipitation - Anti-CCT3 antibody (ab225878)

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (ab225878)

    Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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