AB225878

Anti-CCT3 antibody

Name: Anti-CCT3 antibody (ab225878) | Abcam
Brand: Abcam Limited
SKU: AB225878
Price: 525 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.

View Alternative Names

CCTG, TRIC5, CCT3, T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, Chaperonin containing T-complex polypeptide 1 subunit 3, hTRiC5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : hematoxylin (blue).

Supplier Data

Immunoprecipitation - Anti-CCT3 antibody (AB225878)

CCT3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225878 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225878 at 0.4 μg/ml.

Lane 1 : ab225878 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-CCT3 antibody (ab225878)

Predicted band size: 61 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : hematoxylin (blue).

Supplier Data

Western blot - Anti-CCT3 antibody (AB225878)

All lanes:

Western blot - Anti-CCT3 antibody (ab225878) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human CCT3 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

P49368

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCT3 also known as TCP-1-γ is a subunit of the chaperonin-containing TCP1 (CCT) complex also called the TRiC complex. This protein has a molecular mass of approximately 60 kDa and is expressed in many tissues but is especially abundant in the cytoplasm of eukaryotic cells. CCT3 plays an essential role in the folding of actin and tubulin which are critical for maintaining the cell's cytoskeleton structure. The mechanical function of CCT3 includes providing a folding chamber where newly synthesized polypeptides achieve their correct conformation preventing aggregation and ensuring functional protein production.

Biological function summary

CCT3 is a component of the TRiC/CCT complex which is important for protein quality control within the cell. This complex assists in folding a subset of proteins that are typically difficult to fold without help. CCT3 collaborates with other subunits in the complex to ensure proper assembly and function of structural proteins. Its role is particularly important in high turnover tissues or rapidly proliferating cells where efficient protein folding is essential to maintain cellular function and growth.

Pathways

CCT3 is involved in essential signaling cascades like the cytoskeleton regulation and protein homeostasis pathways. It has relationships with proteins such as actin and beta-tubulin which are critical parts of these pathways. The accurate folding facilitated by CCT3 influences the stability and function of these proteins impacting cell division motility and organization. The involvement in these pathways highlights CCT3’s contribution to maintaining cellular integrity and function.

Mutations or abnormal expression of CCT3 have been linked to neurodegenerative disorders and certain cancers. Dysregulation of this protein may contribute to amyotrophic lateral sclerosis (ALS) due to its role in protein homeostasis and cytoskeleton maintenance. In cancer overexpression of CCT3 has been observed in conditions such as hepatocellular carcinoma potentially promoting tumor growth through altered protein folding. The interaction of CCT3 with proteins like HSP70 during stress responses also connects it to processes that might protect against disease-related protein aggregation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed : 25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

See full target information CCT3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed40077793

2025

Milk Exosome-Based Delivery System for Probiotic Encapsulation That Enhances the Gastrointestinal Resistance and Adhesion of Probiotics.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Hao,Yinxue Liu,Ignatius Man-Yau Szeto,Haining Hao,Tai Zhang,Tongjie Liu,Huaxi Yi

Bioengineered 12:7335-7347 PubMed34612768

2021

Chaperonin containing TCP1 subunit 3 (CCT3) promotes cisplatin resistance of lung adenocarcinoma cells through targeting the Janus kinase 2/signal transducers and activators of transcription 3 (JAK2/STAT3) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Danni,Zeng Jiangzheng,Sun Huamao,Pan Yinglian,Yang Changcheng,Lu Yanda

The world journal of men's health 38:521-534 PubMed31385466

2019

Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin's Disease Prior to Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ana D Martins,Ashok Agarwal,Saradha Baskaran,Peter Natesan Pushparaj,Gulfam Ahmad,Manesh Kumar Panner Selvam

Asian journal of andrology 22:88-93 PubMed31006710

2019

Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia R Dias,Ashok Agarwal,Peter N Pushparaj,Gulfam Ahmad,Rakesh Sharma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

Immunoprecipitation - Anti-CCT3 antibody (AB225878)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

Western blot - Anti-CCT3 antibody (AB225878)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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