Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.
IgG
Rabbit
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Liquid
Polyclonal
IHC-P | IP | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected |
Rat | Predicted | Predicted | Predicted |
Orangutan | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Orangutan | Dilution info - | Notes - |
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Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
CCTG, TRIC5, CCT3, T-complex protein 1 subunit gamma, TCP-1-gamma, CCT-gamma, Chaperonin containing T-complex polypeptide 1 subunit 3, hTRiC5
Rabbit Polyclonal CCT3 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT3 aa 300-350.
IgG
Rabbit
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
ab225878 was affinity purified using an epitope specific to CCT3 immobilized on solid support.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
CCT3 also known as TCP-1-γ is a subunit of the chaperonin-containing TCP1 (CCT) complex also called the TRiC complex. This protein has a molecular mass of approximately 60 kDa and is expressed in many tissues but is especially abundant in the cytoplasm of eukaryotic cells. CCT3 plays an essential role in the folding of actin and tubulin which are critical for maintaining the cell's cytoskeleton structure. The mechanical function of CCT3 includes providing a folding chamber where newly synthesized polypeptides achieve their correct conformation preventing aggregation and ensuring functional protein production.
CCT3 is a component of the TRiC/CCT complex which is important for protein quality control within the cell. This complex assists in folding a subset of proteins that are typically difficult to fold without help. CCT3 collaborates with other subunits in the complex to ensure proper assembly and function of structural proteins. Its role is particularly important in high turnover tissues or rapidly proliferating cells where efficient protein folding is essential to maintain cellular function and growth.
CCT3 is involved in essential signaling cascades like the cytoskeleton regulation and protein homeostasis pathways. It has relationships with proteins such as actin and beta-tubulin which are critical parts of these pathways. The accurate folding facilitated by CCT3 influences the stability and function of these proteins impacting cell division motility and organization. The involvement in these pathways highlights CCT3’s contribution to maintaining cellular integrity and function.
Mutations or abnormal expression of CCT3 have been linked to neurodegenerative disorders and certain cancers. Dysregulation of this protein may contribute to amyotrophic lateral sclerosis (ALS) due to its role in protein homeostasis and cytoskeleton maintenance. In cancer overexpression of CCT3 has been observed in conditions such as hepatocellular carcinoma potentially promoting tumor growth through altered protein folding. The interaction of CCT3 with proteins like HSP70 during stress responses also connects it to processes that might protect against disease-related protein aggregation.
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All lanes: Western blot - Anti-CCT3 antibody (ab225878) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 1s
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
CCT3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225878 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225878 at 0.4 μg/ml.
Lane 1: ab225878 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
All lanes: Immunoprecipitation - Anti-CCT3 antibody (ab225878)
Predicted band size: 61 kDa
Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for CCT3 using ab225878 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).
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