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Rabbit Recombinant Monoclonal CD10 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 9 publications.


Images

Western blot - Anti-CD10 antibody [EPR22867-118] (AB256494), expandable thumbnail
  • Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (AB256494), expandable thumbnail
  • Western blot - Anti-CD10 antibody [EPR22867-118] (AB256494), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (AB256494), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (AB256494), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IFIHC-Fr
Human
Tested
Not recommended
Tested
Tested
Tested
Expected
Mouse
Expected
Not recommended
Tested
Tested
Tested
Tested
Rat
Expected
Not recommended
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Rat

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD10 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22867-118

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.

Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

Associated diseases and disorders

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight observed is consistent with what has been described in the literature (PMID:15286660)

    Exposure time: Lane 1/3: 8 seconds Lane 2: 1 second Lane 4: 3 seconds

    All lanes: Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494) at 1/1000 dilution

    Lane 1: Rat lung tissue lysate at 10 µg

    Lane 2: Rat kidney tissue lysate at 10 µg

    Lane 3: Human tonsil tissue lysate at 10 µg

    Lane 4: Mouse lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

  • Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (ab256494)

    CD10 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab256494 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256494 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10ug

    Lane 2: ab256494 IP in Raji whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab256494 in Raji whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    All lanes: Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Predicted band size: 85 kDa

  • Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    ab256494 was shown to specifically react with CD10 in wild-type HAP1 cells as signal was lost in CD10 knockout cells. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. ab256494 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

    The molecular weight observed is consistent with what has been described in the literature (PMID:15286660) Negative control: HT-29 (PMID:19828468).

    Exposure time: 59 seconds

    All lanes: Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494) at 1/1000 dilution

    Lane 1: Wild type HAP1 whole cell lysate at 20 µg

    Lane 2: CD10 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 4: Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate at 20 µg

    Lane 5: HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Lanes 3 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 85 kDa

    Observed band size: 100 kDa

  • Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CD10 with ab256494 at 1/100 (5.45 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling CD10 with ab256494 at 1:100 (5.45 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1:1000 (2 μg/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunofluorescent analysis of 100% Methanol-fixed 2.4G2 (rat B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in 2.4G2 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunofluorescent analysis of 100% Methanol-fixed WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in WEHI-231 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunofluorescent analysis of 100% Methanol-fixed, Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green).Confocal image showing membranous staining in Ramos cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Negative control: HT-29 (PMID: 19828468)

    Secondary antibody only control: Secondary antibody is ab256494 anti- CD10 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of rat kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of mouse kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on syncytiotrophoblast layer of human placenta (PMID:11092533) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on proximal convoluted tubules and glomerular epithelial cells of human kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

    Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).

    Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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