Anti-CD10 antibody [EPR22867-118] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunofluorescent analysis of 100% Methanol-fixed, Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green).Confocal image showing membranous staining in Ramos cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Negative control : HT-29 （PMID : 19828468）

Secondary antibody only control : Secondary antibody is ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on proximal convoluted tubules and glomerular epithelial cells of human kidney (PMID : 10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on syncytiotrophoblast layer of human placenta (PMID : 11092533)is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-P

AbReview81207****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed human tonsil tissue staining with ab261729 at 1/100 dilution. Secondary antibody was Alexa Fluor™ 555 Donkey anti-Rabbit IgG (H+L). Samples were incubated with the primary antibody with PBS +1% BSA for 16 hours at 4°C. Blocking was done using 5% serum for 1 hour at room temperature. Heat mediated antigen retrieval with EDTA 1mM PH8.

This image is courtesy of an Abreview submitted by Natalie Papazian

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494). Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Unknown

Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

CD10 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab256494 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256494 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10ug

Lane 2 : ab256494 IP in Raji whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab256494 in Raji whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

All lanes:

Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (<a href='/en-us/products/primary-antibodies/cd10-antibody-epr22867-118-ab256494'>ab256494</a>)

Predicted band size: 85 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunofluorescent analysis of 100% Methanol-fixed WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in WEHI-231 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunofluorescent analysis of 100% Methanol-fixed 2.4G2 (rat B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in 2.4G2 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of rat kidney (PMID : 10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling CDK10 with ab256494 at 1/100 (5.45 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1 : 1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of mouse kidney (PMID : 10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (AB261729)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CDK10 with ab256494 at 1/100 (5.45 μg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1 : 1000 (2 μg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1 : 1000 (2 μg/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256494).