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AB34199

Anti-CD10 antibody [SN5c]

1

(1 Review)

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(7 Publications)

Mouse Monoclonal CD10 antibody SN5c - Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

1 Images
Flow Cytometry - Anti-CD10 antibody [SN5c] (AB34199)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD10 antibody [SN5c] (AB34199)

ab34199, Using Neat dilution, Staining of human peripheral blood granulocytes by Flow Cytometry.

  • 660 APC

    APC Anti-CD10 antibody [SN5c]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD10 antibody [SN5c]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SN5c

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing MME protein. The exact immunogen used to generate this antibody is proprietary information.

P08473

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.
Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).
See full target information MME
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 8:95 PubMed36163341

2022

SFRP4 stromal cell subpopulation with IGF1 signaling in human endometrial regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Bingbing Wu,Yu Li,Nanfang Nie,Xilin Shen,Wei Jiang,Yanshan Liu,Lin Gong,Chengrui An,Kun Zhao,Xudong Yao,Chunhui Yuan,Jinghui Hu,Wei Zhao,Jianhua Qian,XiaoHui Zou

International journal of molecular sciences 21: PubMed32012940

2020

Maternal Overweight Downregulates MME (Neprilysin) in Feto-Placental Endothelial Cells and in Cord Blood.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Weiß,Hannah M Berger,Waltraud T Brandl,Jasmin Strutz,Birgit Hirschmugl,Violeta Simovic,Carmen Tam-Ammersdorfer,Silvija Cvitic,Ursula Hiden

Environmental toxicology 33:659-669 PubMed29480568

2018

Niclosamide, an oral antihelmintic drug, exhibits antimetastatic activity in hepatocellular carcinoma cells through downregulating twist-mediated CD10 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Hsien Chien,Yung-Chuan Ho,Shun-Fa Yang,Yi-Chieh Yang,Szu-Yu Lai,Wan-Shen Chen,Ming-Jenn Chen,Chao-Bin Yeh

American journal of physiology. Gastrointestinal a 306:G759-68 PubMed24603459

2014

Overexpression of membrane metalloendopeptidase inhibits substance P stimulation of cholangiocarcinoma growth.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyin Meng,Sharon DeMorrow,Julie Venter,Gabriel Frampton,Yuyan Han,Heather Francis,Holly Standeford,Shanika Avila,Kelly McDaniel,Matthew McMillin,Syeda Afroze,Micheleine Guerrier,Morgan Quezada,Debolina Ray,Lindsey Kennedy,Laura Hargrove,Shannon Glaser,Gianfranco Alpini

PloS one 8:e70721 PubMed23936243

2013

Transcriptional profiling of mRNAs and microRNAs in human bone marrow precursor B cells identifies subset- and age-specific variations.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Jensen,Berit Sletbakk Brusletto,Hans Christian Dalsbotten Aass,Ole K Olstad,Peter Kierulf,Kaare M Gautvik

Leukemia research 13:699-707 PubMed2529399

1989

In vitro and in vivo cytotoxic activity of anti-human leukemia monoclonal antibodies SN5c and SN6 daunorubicin conjugates.

Applications

Unspecified application

Species

Unspecified reactive species

W C Biddle,Y Haruta,B K Seon,E S Henderson,E J Sarcione

Cancer research 47:2160-6 PubMed2435407

1987

Unique epitopes of common acute lymphoblastic leukemia antigen detected by new monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

H Matsuzaki,Y Haruta,T Fukukawa,M P Barcos,B K Seon
View all publications
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