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AB227659

Anti-CD10 antibody [SP179] - N-terminal

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(4 Publications)

Rabbit Recombinant Monoclonal CD10 antibody. N-terminal. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

CD10, EPN, MME, Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neutral endopeptidase 24.11, Skin fibroblast elastase, CALLA, NEP, Neutral endopeptidase, SFE

14 Images
Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Flow cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/20 dilution (7.05 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling CD10 with ab227659 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human placenta tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human prostate tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human kidney tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human liver tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD10 using ab227659 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Flow cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte ) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Flow cytometry analysis of 2.4G2 (Rat B cell lymphoma B lymphocyte) labeling CD10 with purified ab227659 at 1/200 dilution (0.705 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling CD10 with ab227659 at 1/100 dilution (1.41 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-CD10 antibody [SP179] - N-terminal (AB227659)
  • WB

Supplier Data

Western blot - Anti-CD10 antibody [SP179] - N-terminal (AB227659)

All lanes:

Western blot - Anti-CD10 antibody [SP179] - N-terminal (ab227659) at 1/400 dilution

All lanes:

LNCaP (human prostate cancer cell line) cell lysate

Predicted band size: 85 kDa

false

  • Carrier free

    Anti-CD10 antibody [SP179] - BSA and Azide free

  • 660 APC

    APC Anti-CD10 antibody [SP179] (N-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD10 antibody [SP179] - N-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD10 antibody [SP179] - N-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD10 antibody [SP179] - N-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD10 antibody [SP179] - N-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD10 antibody [SP179] - N-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD10 antibody [SP179] - N-terminal

  • HRP

    HRP Anti-CD10 antibody [SP179] (N-terminal)

  • 578 PE

    PE Anti-CD10 antibody [SP179] (N-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP179

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p><p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p><p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Rat": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p><p>Incubate with primary antibody for 10 minutes at room temperature.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD10 also known as neprilysin or neutral endopeptidase is a zinc-dependent metalloprotease enzyme with a mass of approximately 100 kDa. This enzyme is expressed across a variety of tissues including kidney lung and neutrophils. Its expression is also notable in the lymphoid tissues. CD10 functions by cleaving peptides at the N-terminal of hydrophobic residues. Common names used in literature for CD10 include CALLA (common acute lymphoblastic leukemia antigen) and membrane metalloendopeptidase.
Biological function summary

CD10 regulates peptide signaling by inactivating various bioactive peptides such as enkephalins atrial natriuretic peptide and bradykinin. It does not function as part of a complex but rather as a standalone entity. The ability of CD10 to hydrolyze peptides influences signal transmission across cellular membranes impacting cellular communication and signal termination within different systems.

Pathways

Neprilysin plays an essential role in hydrolyzing signaling peptides in several critical pathways. It is especially significant in the natriuretic peptide system and the renin-angiotensin system maintaining blood pressure and fluid balance. Within these pathways CD10 modifies peptides working alongside proteins like atrial natriuretic peptide synthase and angiotensin-converting enzyme ensuring systemic homeostasis and cardiovascular health.

CD10 shows a significant connection to certain types of cancer and Alzheimer's disease. In oncology altered expression of CD10 serves as a CD10 marker in cancers such as lymphomas and some leukemia forms which helps in diagnosis and treatment decisions. Additionally CD10's involvement in neuropeptide degradation links it to Amyloid beta peptide accumulation in Alzheimer's where its dysregulation may promote disease progression. Related proteins are amyloid precursor protein in Alzheimer's and CD19 often co-expressed with CD10 in some lymphomas highlighting its role in both diagnostic and therapeutic contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed : 15283675, PubMed : 6208535, PubMed : 6349683, PubMed : 8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed : 17101991, PubMed : 6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed : 6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed : 15283675, PubMed : 6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed : 16254193, PubMed : 2531377, PubMed : 2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed : 20876573).
See full target information MME
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of pathology and translational medicine 59:149-160 PubMed40468609

2025

Lessons learned from the first 2 years of experience with thyroid core needle biopsy at an Indonesian national referral hospital.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Stephanie Harahap,Maria Francisca Ham,Retno Asti Werdhani,Erwin Danil Julian,Rafi Ilmansyah,Chloe Indira Arfelita Mangunkusumso,Tri Juli Edi Tarigan

PLoS pathogens 20:e1012132 PubMed38620028

2024

Latent Epstein-Barr virus infection collaborates with Myc over-expression in normal human B cells to induce Burkitt-like Lymphomas in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian A Bristol,Scott E Nelson,Makoto Ohashi,Alejandro Casco,Mitchell Hayes,Erik A Ranheim,Abigail S Pawelski,Deo R Singh,Daniel J Hodson,Eric C Johannsen,Shannon C Kenney

Scientific reports 12:19808 PubMed36396721

2022

Sirt3 deficiency induced down regulation of insulin degrading enzyme in comorbid Alzheimer's disease with metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Alpna Tyagi,Musa Musa,Wladimir Labeikovsky,Subbiah Pugazhenthi

Oncology letters 18:1133-1144 PubMed31423173

2019

Cystic and solitary nodular pulmonary metastases in a patient with low-grade endometrial stromal sarcoma: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Xu,Zhi-Xin Liang,Jun-Tang Guo,Xin Su,Yun-Long Lu,Xi-Zhou Guan
View all publications
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