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AB192342

Anti-CD101 antibody [BB27]

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(1 Publication)

Mouse Monoclonal CD101 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD101 protein.

View Alternative Names

CD101, EWI101, IGSF2, V7, Immunoglobulin superfamily member 2, IgSF2, Cell surface glycoprotein V7, Glu-Trp-Ile EWI motif-containing protein 101, EWI-101

3 Images
Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)

Separation of human CD101 positive CD16 negative lymphocytes (red-filled) CD101 negative CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab192342 (concentration in sample 0.56 μg/ml, GAM FITC).

Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab192342 (concentration in sample 0.56 μg/ml, GAM FITC).

Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD101 antibody [BB27] (AB192342)

Flow cytometry multicolor surface staining of human lymphocytes stained using ab192342 (concentration in sample 0.56 μg/ml, GAM FITC) and anti-human CD16 (3G8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

  • 578 PE

    PE Anti-CD101 antibody [BB27]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BB27

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD101 protein. The exact immunogen used to generate this antibody is proprietary information.

Q93033

Specificity

The mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfidebonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-4 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab192342 was purified from hybridoma culture supernatant by protein-A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD101 also known as IGSF2 or V7 is a transmembrane protein that weighs approximately 140 kDa. This protein is part of the immunoglobulin superfamily and is expressed on the surface of certain immune cells including dendritic cells macrophages monocytes and regulatory T cells. Researchers have also detected CD101 on some subsets of B cells. The protein has a significant role in the modulation of immune responses and functions as a marker in immune system studies.
Biological function summary

CD101 influences the immune response through its regulatory effects on cell signaling and interaction. The protein's function often centers within a complex environment contributing to the inhibitory signaling pathways that help balance immune activation. Its presence on regulatory T cells suggests a role in suppressing overactive immune responses which is important to maintaining immune homeostasis. Researchers associate CD101 expression with the control of inflammatory processes highlighting its functional significance.

Pathways

Research shows that CD101 participates in pathways key to immune regulation such as the T-cell receptor signaling pathway. This involvement connects CD101 with proteins like CD3 and CD4 emphasizing its role in modulating T-cell response. The protein plays a part in downstream signaling events that fine-tune immune responses by dampening excessive activation therefore helping prevent autoimmune reactions. It ensures the immune system effectively targets pathogens without harming host tissues.

Studies correlate CD101 expression with autoimmune diseases and inflammatory diseases including inflammatory bowel disease (IBD). These associations suggest a potential protective role of CD101 in managing chronic inflammation. Scientists have also observed interactions with proteins like TNF-alpha in these contexts reflecting the complex interplay CD101 has with pro-inflammatory cytokines. Understanding CD101's involvement in disease processes can aid in developing therapeutic strategies that aim to modulate immune responses effectively.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.
See full target information CD101

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 23:90 PubMed27931227

2016

Epigenetics of amphetamine-induced sensitization: HDAC5 expression and microRNA in neural remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Philip K Liu,Christina H Liu
View all publications

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