JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB25198

Anti-CD103 antibody [2E7]

Be the first to review this product! Submit a review

|

(1 Publication)

Armenian hamster Monoclonal CD103 antibody. Suitable for IP, Flow Cyt, FuncS (Act), IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD103, Integrin alpha-E, Integrin alpha M290, Itgae

  • Biotin

    Biotin Anti-CD103 antibody [2E7]

  • 519 FITC

    FITC Anti-CD103 antibody [2E7]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

2E7

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, IP, Flow Cyt, FuncS (Act)

applications

style
left-column

Alternative Products

Primary Antibodies

AB227697

Anti-CD103 antibody [SP301]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [SP301] (AB227697)

  • 0
  • 0
Primary Antibodies

AB108503

Anti-CD103 antibody [EPR4166]

RabMAb|Recombinant

Western blot - Anti-CD103 antibody [EPR4166] (AB108503)

  • 0
  • 0
Primary Antibodies

AB129202

Anti-CD103 antibody [EPR4166(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] (AB129202)

  • 5
  • 8
Primary Antibodies

AB221210

Anti-CD103 antibody [EPR4166(2)] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [EPR4166(2)] - Low endotoxin, Azide free (AB221210)

  • 1
  • 1
Primary Antibodies

AB225153

Alexa Fluor® 647 Anti-CD103 antibody [EPR4166(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD103 antibody [EPR4166(2)] (AB225153)

  • 4
  • 2
Primary Antibodies

AB245746

Anti-CD103 antibody [SP301] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB307081

Anti-CD69 antibody [EPR25398-81]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CD69 antibody [EPR25398-81] (AB307081)

  • 0
  • 0
Primary Antibodies

AB217344

Anti-CD8 alpha antibody [EPR21769]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD8 alpha antibody [EPR21769] (AB217344)

  • 5
  • 6
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "FuncSAct-species-checked": "guaranteed", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

Product details

This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 8.2 Constituents: 0.95% Sodium borate, 0.6% Boric Acid
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.
Biological function summary

CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.

Pathways

CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.

CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integrin ITGAE/ITGB7 (alpha-E/beta-7) is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
See full target information Itgae
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 15:4979-4986 PubMed29805521

2018

Prognostic and therapeutic value of CD103 cells in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhou,Lipeng Liu,Tuo Yang,Bingxin Lu
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com