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AB227697

Anti-CD103 antibody [SP301]

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(6 Publications)

Rabbit Recombinant Monoclonal CD103 antibody. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD103, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, ITGAE

17 Images
Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [SP301] (AB227697)

IHC image of CD103 staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab227697, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD103 antibody [SP301] (AB227697)

IHC image of CD103 staining in a section of frozen normal human colon* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab227697, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human stomach tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human stomach adenocarcinoma carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human thymus tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human colon adenocarcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human breast tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human colon tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] (AB227697)

Formalin-fixed paraffin-embedded human cervix tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry - Anti-CD103 antibody [SP301] (AB227697)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD103 antibody [SP301] (AB227697)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD103 with ab227697 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP301

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p>Incubate with primary antibody for 30 minutes at 4 °C.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Incubate for 10 minutes at room temperature.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "10 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.
Biological function summary

CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.

Pathways

CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.

CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAE/ITGB7 (alpha-E/beta-7) is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
See full target information ITGAE
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0322708 PubMed40367036

2025

Overexpression of LINC00672 promotes autophagy in Alzheimer's disease by upregulating GPNMB.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyi Gao,Shijun Hu,Yan Lv,Guoxian Zheng,Zhichuan Lin

Cell reports. Medicine 5:101852 PubMed39657664

2024

Lurbinectedin sensitizes PD-L1 blockade therapy by activating STING-IFN signaling in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Subhamoy Chakraborty,Utsav Sen,Kedwin Ventura,Vrinda Jethalia,Charles Coleman,Subhasree Sridhar,Avisek Banerjee,Hilal Ozakinci,Yazhini Mahendravarman,Konrad Snioch,Elisa de Stanchina,Misty D Shields,Lewis E Tomalin,Deniz Demircioglu,Theresa A Boyle,Anna Tocheva,Dan Hasson,Triparna Sen

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4601-4608 PubMed39167623

2024

Pembrolizumab and Cabozantinib in Recurrent and/or Metastatic Head and Neck Squamous Cell Carcinoma: Long-term Survival Update with a Biomarker Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nabil F Saba,Ritu Chaudhary,Kedar Kirtane,Angelo Marra,Asari Ekpenyong,Ashley McCook-Veal,Nicole C Schmitt,Jennifer H Gross,Mihir R Patel,Jill Remick,James E Bates,Mark W McDonald,Soumon F Rudra,William A Stokes,Maria Biernacki,Xiaofei Song,Robbert J C Slebos,Yuan Liu,Conor E Steuer,Dong M Shin,Yong Teng,Christine H Chung

Nature biomedical engineering 8:345-360 PubMed38114742

2023

Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids.

Applications

Unspecified application

Species

Unspecified reactive species

Marius F Harter,Timothy Recaldin,Regine Gerard,Blandine Avignon,Yannik Bollen,Cinzia Esposito,Karolina Guja-Jarosz,Kristina Kromer,Adrian Filip,Julien Aubert,Anneliese Schneider,Marina Bacac,Michael Bscheider,Nadine Stokar-Regenscheit,Salvatore Piscuoglio,Joep Beumer,Nikolche Gjorevski

Cancer research communications 3:896-907 PubMed37377902

2023

EGFR Inhibition by Cetuximab Modulates Hypoxia and IFN Response Genes in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Chaudhary,Robbert J C Slebos,Leenil C Noel,Feifei Song,Maria I Poole,Dirk S Hoening,Juan C Hernandez-Prera,Jose R Conejo-Garcia,Jose A Guevara-Patino,Xuefeng Wang,Mengyu Xie,Aik Choon Tan,Christine H Chung

Cancer cell 40:545-557.e13 PubMed35427494

2022

Ovarian cancer immunogenicity is governed by a narrow subset of progenitor tissue-resident memory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen M Anadon,Xiaoqing Yu,Kay Hänggi,Subir Biswas,Ricardo A Chaurio,Alexandra Martin,Kyle K Payne,Gunjan Mandal,Patrick Innamarato,Carly M Harro,Jessica A Mine,Kimberly B Sprenger,Carla Cortina,John J Powers,Tara Lee Costich,Bradford A Perez,Chandler D Gatenbee,Sandhya Prabhakaran,Douglas Marchion,Mirjam H M Heemskerk,Tyler J Curiel,Alexander R Anderson,Robert M Wenham,Paulo C Rodriguez,Jose R Conejo-Garcia
View all publications
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Product promise

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