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AB245746

Anti-CD103 antibody [SP301] - BSA and Azide free

4

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal CD103 antibody. Carrier free. Suitable for Flow Cyt, IHC-P, IHC-Fr and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD103, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, ITGAE

15 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227697).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human cervix tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227697).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human breast tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling CD103 with ab227697 at 1/100 dilution (3.99 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human stomach tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD103 with ab227697 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS#44; BSA#44; and sodium azide (ab227697)

Flow Cytometry - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD103 antibody [SP301] - BSA and Azide free (AB245746)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD103 with ab227697 at 1/100 dilution (green) compared with a negative control rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227697).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP301

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-Fr, Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Incubate with primary antibody for 30 minutes at 4°C.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate for 10 minutes at room temperature.</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "10 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab245746 is the carrier-free version of ab227697.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.
Biological function summary

CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.

Pathways

CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.

CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the ITGAE gene, known as integrin alpha-E/beta-7, functions as a receptor for E-cadherin and mediates the adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. This supplementary information is collated from multiple sources and compiled automatically.
See full target information ITGAE
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 42:396-412.e5 PubMed38242124

2024

Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Cords,Stefanie Engler,Martina Haberecker,Jan Hendrik Rüschoff,Holger Moch,Natalie de Souza,Bernd Bodenmiller

iScience 25:103785 PubMed35146396

2022

PD-L1CD8 T cells enrichment in lung cancer exerted regulatory function and tumor-promoting tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxia Zheng,Li Han,Zheyi Chen,Yiyang Li,Bingqian Zhou,Rui Hu,Shiyu Chen,Haibo Xiao,Yanhui Ma,Guohua Xie,Junyao Yang,Xianting Ding,Lisong Shen

Cell reports. Medicine 2:100166 PubMed33521697

2021

A distinct innate immune signature marks progression from mild to severe COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Chevrier,Yves Zurbuchen,Carlo Cervia,Sarah Adamo,Miro E Raeber,Natalie de Souza,Sujana Sivapatham,Andrea Jacobs,Esther Bachli,Alain Rudiger,Melina Stüssi-Helbling,Lars C Huber,Dominik J Schaer,Jakob Nilsson,Onur Boyman,Bernd Bodenmiller
View all publications
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