AB314101

Anti-CD103/ITGAE antibody [BLR171J] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal CD103 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ITGAE aa 400-450.

View Alternative Names

CD103, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, ITGAE

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Detection of human CD103/ITGAE in FFPE breast carcinoma by IHC. Antibody : Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Detection of human CD103/ITGAE in FFPE C5/MJ cells by ICC. Antibody : Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Detection of human CD103/ITGAE in FFPE tonsil by IHC. Antibody : Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary : HRP-conjugated goat anti-rabbit IgG. Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies : Rabbit antiCD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171) used for IP at 6 µl/mg lysate. CD103/ITGAE was also immunoprecipitated by a second antibody against a different epitope of CD103/ITGAE (Antibody 2). For blotting immunoprecipitated CD103/ITGAE, A700-171 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101) at 1/1000 dilution

All lanes:

MJ cells at 1 mg

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Supplier Data

Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Samples : Whole cell lysate (50 µg) from C5/MJ, HeLa, CCRF-CEM, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody : Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171) used at 1 : 1000. Secondary : HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit antiCOPB2 antibody.

All lanes:

Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101) at 1/1000 dilution

Lane 1:

C5/MJ Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

CCRF-CEM Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

MJ Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat antirabbit IgG

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR171J

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human ITGAE aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

P38570

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.

Biological function summary

CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.

Pathways

CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.

CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the ITGAE gene, known as integrin alpha-E/beta-7, functions as a receptor for E-cadherin and mediates the adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. This supplementary information is collated from multiple sources and compiled automatically.

See full target information ITGAE

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD103/ITGAE antibody [BLR171J] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise