Rabbit Recombinant Monoclonal CD103 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ITGAE aa 400-450.
Images
![Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--western-blot-img420656.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)")
![Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunocytochemistry-img420660.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420658.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)")
![Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunoprecipitation-img420657.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (AB314101)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human ITGAE aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P38570
Reactivity data
Select an application| ICC | IHC-P | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Alternative names
CD103, Integrin alpha-E, HML-1 antigen, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen, ITGAE
Recommended products
Rabbit Recombinant Monoclonal CD103 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ITGAE aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human ITGAE aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P38570
- Clone number
- BLR171J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD103 also known as ITGAE or integrin alpha E is a protein subunit that pairs with integrin beta 7 (ItgB7) to form a complete heterodimer known as the αEβ7 integrin complex. This protein typically weighs around 150 kDa. CD103 is mainly expressed on the surface of certain T lymphocytes such as CD8+ T cells in mucosal tissues and also in a subset of dendritic cells. Through its mechanical function CD103 mediates cell adhesion by specifically binding to E-cadherin on epithelial cells. This interaction facilitates the retention and positioning of lymphocytes in epithelial tissues.
- Biological function summary
CD103 is essential for establishing and maintaining interactions between immune cells and epithelial cells. By incorporating into the αEβ7 integrin complex it plays an important role in the localization of intraepithelial lymphocytes and dendritic cells in the gut mucosa and skin contributing to immune surveillance and protection of these barriers. CD103 helps to control lymphocyte activation and the migration process offering a critical surface marker for identifying tissue-resident memory T cells (Trm cells) and discerning their functional potential.
- Pathways
CD103 functions prominently in the immunological environment by participating in pathways that regulate lymphocyte adherence and migration. Two significant pathways it fits into include the T-cell homing and retention pathways and the antigen presentation pathway. Through these pathways CD103 interacts with molecules such as E-cadherin and impacts the movement of T cells to epithelial sites influencing tissue-specific immune responses alongside related proteins like integrin beta 7 which forms a part of the same integrin complex.
- Associated diseases and disorders
CD103 has a significant connection with inflammatory and autoimmune conditions particularly in the context of inflammatory bowel disease and certain types of cancer such as colorectal cancer. Its association with E-cadherin through the integrin complex affects cellular behavior in these diseases contributing to either barrier integrity or breakdown and abnormal immune cell infiltration. Additionally in the disease context CD103 can influence interactions with proteins that regulate immune responses and tumor surveillance highlighting its role in disease progression and therapeutic potential.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--western-blot-img420656.jpg "Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)")
Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)
Samples: Whole cell lysate (50 µg) from C5/MJ, HeLa, CCRF-CEM, HEK293T, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171) used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit antiCOPB2 antibody.
All lanes: Western blot - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101) at 1/1000 dilution
Lane 1: C5/MJ Whole cell lysate at 50 µg
Lane 2: HeLa Whole cell lysate at 50 µg
Lane 3: CCRF-CEM Whole cell lysate at 50 µg
Lane 4: HEK293T Whole cell lysate at 50 µg
Lane 5: MJ Whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat antirabbit IgG
![Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunocytochemistry-img420660.jpg "Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)")
Immunocytochemistry - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)
Detection of human CD103/ITGAE in FFPE C5/MJ cells by ICC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)
Detection of human CD103/ITGAE in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img420658.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)
Detection of human CD103/ITGAE in FFPE tonsil by IHC. Antibody: Rabbit anti-CD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
![Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101), expandable thumbnail](https://content.abcam.com/products/images/cd103-itgae-antibody-blr171j-bsa-free-ab314101--immunoprecipitation-img420657.jpg "Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)")
Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ cells prepared using NETN lysis buffer. Antibodies: Rabbit antiCD103/ITGAE recombinant monoclonal antibody [BLR171J] (A700-171) used for IP at 6 µl/mg lysate. CD103/ITGAE was also immunoprecipitated by a second antibody against a different epitope of CD103/ITGAE (Antibody 2). For blotting immunoprecipitated CD103/ITGAE, A700-171 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-CD103/ITGAE antibody [BLR171J] - BSA free (ab314101) at 1/1000 dilution
All lanes: MJ cells at 1 mg
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