Mouse Monoclonal CD105 antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Human ENG.
Images
![Western blot - Anti-CD105 antibody [105C02] (AB44967), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-105c02-ab44967--western-blot-img2515.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD105 antibody [105C02] (AB44967)")
Publications
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- Bioengineering (Basel, Switzerland) 9:2022Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlois Hopf,Lina Al-Bayati,Dirk J Schaefer,Daniel F Kalbermatten,Raphael Guzman,Srinivas MadduriPubMed 36134958
- Histochemistry and cell biology 156:349-3622021Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamil Wartalski,Gabriela Gorczyca,Jerzy Wiater,Zbigniew Tabarowski,Małgorzata DudaPubMed 34269874
- Experimental and therapeutic medicine 20:1122020Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun Pan et. al.PubMed 32989390
- Nature communications 11:10642020Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPhuong-Uyen C Dinh et. al.PubMed 32111836
- Stem cells international 2019:15167462019Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonella Bordin et. al.PubMed 30755774
- International journal of molecular medicine 43:890-9002018Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi-Qiang Zhou,Yi Chen,Mi Chai,Ran Tao,Yong-Hong Lei,Yi-Qing Jia,Jun Shu,Jing Ren,Guo Li,Wen-Xin Wei,Yu-Di Han,Yan HanPubMed 30535488
- Cell transplantation 25:1287-12972017A Simple, Rapid, and Efficient Method for Isolating Mesenchymal Stem Cells from the Entire Umbilical Cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMehdi Banitalebi Dehkordi,Zahra Madjd,Morteza Hashemzadeh Chaleshtori,Reza Meshkani,Laleh Nikfarjam,Abdol-Mohammad KajbafzadehPubMed 28836831
- Biomedical reports 6:519-5242017Biocompatibility of biological material polylactic acid with stem cells from human exfoliated deciduous teeth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Wang,Guanghui Li,Yiming Liu,Weiwei Yu,Qiang SunPubMed 28515910
- Stem cell research 17:342-3512016The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCamilla Siciliano et. al.PubMed 27614132
- Neurology research international 2014:8078962014Differentiation of human breast-milk stem cells to neural stem cells and neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeyed Mojtaba Hosseini et. al.PubMed 25506428
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Full Length Protein corresponding to Human ENG. The exact immunogen used to generate this antibody is proprietary information. Database link P17813
Consider this alternative
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
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7 products for Alternative Product
Target data
Function
Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529, PubMed:8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).
Alternative names
CD105, END, ENG, Endoglin
Recommended products
Mouse Monoclonal CD105 antibody. Suitable for WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Full Length Protein corresponding to Human ENG.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Full Length Protein corresponding to Human ENG. The exact immunogen used to generate this antibody is proprietary information. Database link P17813
- Clone number
- 105C02
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
- Biological function summary
Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.
- Pathways
CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.
- Associated diseases and disorders
CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-CD105 antibody [105C02] (ab44967), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-105c02-ab44967--western-blot-img2515.jpg "Western blot - Anti-CD105 antibody [105C02] (ab44967)")
Western blot - Anti-CD105 antibody [105C02] (ab44967)
Lanes 1 - 3: Merged signal (red and green). Green - ab44967 observed at 95 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
ab44967 was shown to specifically react with CD105 in wild-type HeLa cells as signal was lost in CD105 knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab44967 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD105 antibody [105C02] (ab44967) at 1/1000 dilution
Lane 1: Wild-type HeLa whole cell lysate at 20 µg
Lane 2: CD105 knockout HeLa whole cell lysate at 20 µg
Lane 3: MCF7 whole cell lysate at 20 µg
Predicted band size: 70 kDa
Observed band size: 95 kDa
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