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AB44967

Anti-CD105 antibody [105C02]

4

(2 Reviews)

|

(13 Publications)

Mouse Monoclonal CD105 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Full Length Protein corresponding to Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

1 Images
Western blot - Anti-CD105 antibody [105C02] (AB44967)
  • WB

Lab

Western blot - Anti-CD105 antibody [105C02] (AB44967)

Lanes 1 - 3 : Merged signal (red and green). Green - ab44967 observed at 95 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab44967 was shown to specifically react with CD105 in wild-type HeLa cells as signal was lost in CD105 knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab44967 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD105 antibody [105C02] (ab44967) at 1/1000 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 20 µg

Lane 2:

CD105 knockout HeLa whole cell lysate at 20 µg

Lane 3:

MCF7 whole cell lysate at 20 µg

Predicted band size: 70 kDa

Observed band size: 95 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

105C02

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Full Length Protein corresponding to Human ENG. The exact immunogen used to generate this antibody is proprietary information.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Folia morphologica 83:72-82 PubMed36967622

2023

Biodegradable nanofiber coated human umbilical cord as nerve scaffold for sciatic nerve regeneration in albino Wistar rats.

Applications

Unspecified application

Species

Unspecified reactive species

Balasundari Ramesh,Jaikanth Chandrasekaran,Kotturathu Mammen Cherian,Adegbenro Omotuyi John Fakoya

Bioengineering (Basel, Switzerland) 9: PubMed36134958

2022

Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering.

Applications

Unspecified application

Species

Unspecified reactive species

Alois Hopf,Lina Al-Bayati,Dirk J Schaefer,Daniel F Kalbermatten,Raphael Guzman,Srinivas Madduri

Histochemistry and cell biology 156:349-362 PubMed34269874

2021

Porcine ovarian cortex-derived putative stem cells can differentiate into endothelial cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Kamil Wartalski,Gabriela Gorczyca,Jerzy Wiater,Zbigniew Tabarowski,Małgorzata Duda

Experimental and therapeutic medicine 20:112 PubMed32989390

2020

Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Pan,Xu Zheng,Liang Wang,Qian Chen,Qi Lin

Nature communications 11:1064 PubMed32111836

2020

Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong-Uyen C Dinh,Dipti Paudel,Hayden Brochu,Kristen D Popowski,M Cyndell Gracieux,Jhon Cores,Ke Huang,M Taylor Hensley,Erin Harrell,Adam C Vandergriff,Arianna K George,Raina T Barrio,Shiqi Hu,Tyler A Allen,Kevin Blackburn,Thomas G Caranasos,Xinxia Peng,Lauren V Schnabel,Kenneth B Adler,Leonard J Lobo,Michael B Goshe,Ke Cheng

Stem cells international 2019:1516746 PubMed30755774

2019

Oral Plaque from Type 2 Diabetic Patients Reduces the Clonogenic Capacity of Dental Pulp-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Bordin,Francesca Pagano,Eleonora Scaccia,Matteo Saccucci,Iole Vozza,Noemi Incerti,Antonella Polimeni,Elena Cavarretta,Isotta Chimenti,Elena De Falco

International journal of molecular medicine 43:890-900 PubMed30535488

2018

Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Qiang Zhou,Yi Chen,Mi Chai,Ran Tao,Yong-Hong Lei,Yi-Qing Jia,Jun Shu,Jing Ren,Guo Li,Wen-Xin Wei,Yu-Di Han,Yan Han

Cell transplantation 25:1287-1297 PubMed28836831

2017

A Simple, Rapid, and Efficient Method for Isolating Mesenchymal Stem Cells from the Entire Umbilical Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Banitalebi Dehkordi,Zahra Madjd,Morteza Hashemzadeh Chaleshtori,Reza Meshkani,Laleh Nikfarjam,Abdol-Mohammad Kajbafzadeh

Biomedical reports 6:519-524 PubMed28515910

2017

Biocompatibility of biological material polylactic acid with stem cells from human exfoliated deciduous teeth.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wang,Guanghui Li,Yiming Liu,Weiwei Yu,Qiang Sun

Stem cell research 17:342-351 PubMed27614132

2016

The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots.

Applications

Unspecified application

Species

Unspecified reactive species

Camilla Siciliano,Antonella Bordin,Mohsen Ibrahim,Isotta Chimenti,Francesco Cassiano,Ilenia Gatto,Giorgio Mangino,Andrea Coccia,Selenia Miglietta,Daniela Bastianelli,Vincenzo Petrozza,Antonella Calogero,Giacomo Frati,Elena De Falco
View all publications
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