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AB230925

Anti-CD105 antibody [8A1]

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(7 Publications)

Mouse Monoclonal CD105 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Dog, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (AB230925)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (AB230925)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD105 using ab230925 at 10 μg/ml in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (AB230925)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (AB230925)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD105 (green) using ab230925 at 1/100 dilution in ICC/IF.

Western blot - Anti-CD105 antibody [8A1] (AB230925)
  • WB

Supplier Data

Western blot - Anti-CD105 antibody [8A1] (AB230925)

All lanes:

Western blot - Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 3:

SVT2 whole cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) whole cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

Predicted band size: 70 kDa

true

Western blot - Anti-CD105 antibody [8A1] (AB230925)
  • WB

Supplier Data

Western blot - Anti-CD105 antibody [8A1] (AB230925)

All lanes:

Western blot - Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY CD105-transfected HEK-293T whole cell lysate at 5 µg

Predicted band size: 70 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8A1

Isotype

IgG2b

Carrier free

No

Reacts with

Rat, Dog, Human, African green monkey, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ENG.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/µL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/µL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70140 PubMed39434203

2024

Hoxc10-mediated 'positional memory' regulates cartilage formation subsequent to femoral heterotopic grafting.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Song,Yujia Hao,Qingpeng Xie,Xiaohang Chen,Na Li,Jia Wang,Xiaoxuan Zhang,Yuan Zhang,Jinjia Hong,Shuyun Xue,Pengfei Zhang,Si Xie,Xing Wang

International journal of molecular sciences 24: PubMed37511521

2023

Esaxerenone Inhibits Renal Angiogenesis and Endothelial-Mesenchymal Transition via the VEGFA and TGF-β1 Pathways in Aldosterone-Infused Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomeng Gao,Jingyue Chang,Yi Chang,Lili Fan,Ziqian Liu,Cuijuan Zhang,Tatsuo Shimosawa,Fan Yang,Qingyou Xu

Turkish neurosurgery 33:162-170 PubMed36482856

2022

The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ziya Yurtal,Huseyin Ozkan,Tuncer Kutlu,Mehmet Deveci,Boran Urfali,Senem Urfali

Cell proliferation 56:e13366 PubMed36478274

2022

CD34 cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Soo-Been Jeon,A-Reum Han,Sunghun Lee,Seung Chan Lee,Min Ji Lee,Soon-Jung Park,Sung-Hwan Moon,Ji Yoon Lee

Theranostics 11:6393-6406 PubMed33995664

2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect .

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhong,Wei Shi,Lu Gan,Xiuli Liu,Yu Huo,Pan Wu,Zhikun Zhang,Tao Wu,Hongmei Peng,Yong Huang,Yongxiang Zhao,Yulin Yuan,Zhiming Deng,Hongliang Tang

Journal of clinical medicine 9: PubMed32486396

2020

Allogeneic Bone Marrow Mesenchymal Stem Cell Transplantation in Tooth Extractions Sites Ameliorates the Incidence of Osteonecrotic Jaw-Like Lesions in Zoledronic Acid-Treated Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco Javier Rodríguez-Lozano,Ricardo Oñate-Sánchez,Mar Gonzálvez-García,Marta Vallés-Bergadá,Carlos M Martínez,Beatriz Revilla-Nuin,Julia Guerrero-Gironés,Jose M Moraleda,David García-Bernal

Stem cells and development 26:374-389 PubMed27881051

2017

Mechanisms of Immune Suppression Utilized by Canine Adipose and Bone Marrow-Derived Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lyndah Chow,Valerie Johnson,Jonathan Coy,Dan Regan,Steven Dow
View all publications
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