Skip to main content

Mouse Monoclonal CD105 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Dog, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENG.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (AB230925), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (AB230925), expandable thumbnail
  • Western blot - Anti-CD105 antibody [8A1] (AB230925), expandable thumbnail
  • Western blot - Anti-CD105 antibody [8A1] (AB230925), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human ENG. Database link P17813

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Rat
Expected
Expected
Expected
African green monkey
Expected
Expected
Expected
Dog
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
10 µg/µL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
African green monkey
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Dog
Dilution info
10 µg/µL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/200 - 1/4000
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
1/200 - 1/4000
Notes

-

Species
Dog
Dilution info
1/200 - 1/4000
Notes

-

Species
Mouse
Dilution info
1/200 - 1/4000
Notes

-

Species
Rat
Dilution info
1/200 - 1/4000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
1/100
Notes

-

Species
Dog
Dilution info
1/100
Notes

-

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529, PubMed:8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).

Alternative names

CD105, END, ENG, Endoglin

Recommended products

Mouse Monoclonal CD105 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Dog, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENG.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human ENG. Database link P17813
Clone number
8A1
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

Associated diseases and disorders

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (ab230925), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [8A1] (ab230925)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD105 using ab230925 at 10 μg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (ab230925), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [8A1] (ab230925)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CD105 (green) using ab230925 at 1/100 dilution in ICC/IF.

  • Western blot - Anti-CD105 antibody [8A1] (ab230925), expandable thumbnail

    Western blot - Anti-CD105 antibody [8A1] (ab230925)

    All lanes: Western blot - Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

    Lane 1: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 35 µg

    Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

    Lane 3: SVT2 whole cell lysate at 35 µg

    Lane 4: A549 (human lung carcinoma cell line) whole cell lysate at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 35 µg

    Lane 6: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg

    Lane 7: MDCK (canine kidney cell line) whole cell lysate at 35 µg

    Lane 8: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 35 µg

    Lane 9: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa

  • Western blot - Anti-CD105 antibody [8A1] (ab230925), expandable thumbnail

    Western blot - Anti-CD105 antibody [8A1] (ab230925)

    All lanes: Western blot - Anti-CD105 antibody [8A1] (ab230925) at 1/200 dilution

    Lane 1: pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg

    Lane 2: pCMV6-ENTRY CD105-transfected HEK-293T whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com