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AB137389

Anti-CD105 antibody

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(4 Publications)

Rabbit Polyclonal CD105 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ENG aa 300-650.

View Alternative Names

CD105, END, ENG, Endoglin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody (AB137389)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody (AB137389)

Immunohistochemical analysis of paraffin-embedded Human SCC15 xenograft tissue, labelling CD105 with ab137389 at 1/100 dilution.

Western blot - Anti-CD105 antibody (AB137389)
  • WB

Supplier Data

Western blot - Anti-CD105 antibody (AB137389)

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 antibody (ab137389) diluted at 1 : 1000.

All lanes:

Western blot - Anti-CD105 antibody (ab137389) at 1/1000 dilution

All lanes:

HUVEC whole cell extract at 30 µg

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ENG aa 300-650. The exact immunogen used to generate this antibody is proprietary information.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer (pH8.0)</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell and tissue research 384:613-624 PubMed33471197

2021

Endoglin and TGF-β signaling in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Burghardt,Elisa Ventura,Tobias Weiss,Judith Johanna Schroeder,Katharina Seystahl,Christian Zielasek,Dorothee Gramatzki,Michael Weller

Cancer biology & medicine 17:418-432 PubMed32587778

2020

Bevacizumab promotes active biological behaviors of human umbilical vein endothelial cells by activating TGFβ1 pathways off-VEGF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Zhang,Yan Zhang,Yanan Jia,Tingting Qin,Cuicui Zhang,Yueya Li,Chengmou Huang,Zhujun Liu,Jing Wang,Kai Li

Stem cells international 2019:1916542 PubMed30809262

2019

Angiogenic Properties of Menstrual Stem Cells Are Impaired in Women with a History of Preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Varas-Godoy,Stephanie Acuña-Gallardo,Sebastian Venegas-Duarte,Charlotte Hill,Albano Caceres-Verschae,Ornella Realini,Lara J Monteiro,Gabriela Zavala,Maroun Khoury,Roberto Romero,Gregory Rice,Sebastian E Illanes

Oncotarget 6:22480-95 PubMed26090865

2015

Modulation of cerebral endothelial cell function by TGF-β in glioblastoma: VEGF-dependent angiogenesis versus endothelial mesenchymal transition.

Applications

WB

Species

Unspecified reactive species

Shanmugarajan Krishnan,Emese Szabo,Isabel Burghardt,Karl Frei,Ghazaleh Tabatabai,Michael Weller
View all publications
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