Skip to main content

Rabbit Recombinant Monoclonal CD105 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications.


Images

Western blot - Anti-CD105 antibody [EPR10145-12] (AB169545), expandable thumbnail
  • Western blot - Anti-CD105 antibody [EPR10145-12] (AB169545), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (AB169545), expandable thumbnail
  • Western blot - Anti-CD105 antibody [EPR10145-12] (AB169545), expandable thumbnail
  • Western blot - Anti-CD105 antibody [EPR10145-12] (AB169545), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/2000

Notes

For unpurified use at 1/50. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/900

Notes

For unpurified use at 1/30.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

15 products for Alternative Product

1 product for Alternative Version

Target data

Function

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:8370410, PubMed:21737454, PubMed:22347366, PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).

Alternative names

Endoglin, ENG, END

Recommended products

Rabbit Recombinant Monoclonal CD105 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 19 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10145-12

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in HeLa wild type cells. Loss of signal was observed when knockout cell line Human ENG (CD105) knockout HeLa cell line ab265178 (knockout cell lysate Human ENG (CD105) knockout HeLa cell lysate ab256906) was used. Wild type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ENG knockout HeLa cell lysate at 20 µg

    Lane 3: HUVEC cell lysate at 20 µg

    Lane 4: MCF7 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 70 kDa

    Observed band size: 70-120 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Lanes 1 - 3: Merged signal (red and green). Green - ab169545 observed at 70 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab169545 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

    Lane 1: Wild-type HeLa whole cell lysate at 20 µg

    Lane 2: CD105 knockout HeLa whole cell lysate at 20 µg

    Lane 3: HUVEC whole cell lysate at 20 µg

    Predicted band size: 70 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with unpurified ab169545 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

    Lane 1: ECV-304 cell lysate at 10 µg

    Lane 2: Human tonsil cell lysate at 10 µg

    Lane 3: HUVEC cell lysate at 10 µg

    Secondary

    All lanes: HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 70 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution

    All lanes: ECV-304 cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution

    All lanes: ECV-304 cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 45 kDa, 70 kDa

    Observed band size: 40 kDa, 77 kDa, 95 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

    All lanes: immunoprecipitation pellet from ECV-304 cell lysate

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 70 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution

    All lanes: HUVEC cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa

    Observed band size: 95 kDa

  • Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution

    All lanes: HUVEC cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 70 kDa

    Observed band size: 95 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with purified ab169545 at 1/900. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human clear cell carcinoma tissue labelling CD105 with unpurified ab169545 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling CD105 with unpurified ab169545 at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD105 with unpurified ab169545 at 1/250.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com