Anti-CD105 antibody [EPR19911] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] - BSA and Azide free (AB251457)

This data was developed using ab206419, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm on human tonsil vascular endothelium is observed [PMID : 17502949].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] - BSA and Azide free (AB251457)

This data was developed using ab206419, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm on human kidney vascular endothelium is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CD105 antibody [EPR19911] - BSA and Azide free (AB251457)

This data was developed using ab206419, the same antibody clone in a different buffer formulation.

Lanes 1 - 2 : Merged signal (red and green). Green - ab206419 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab206419 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab206419 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD105 antibody [EPR19911] (<a href='/en-us/products/primary-antibodies/cd105-antibody-epr19911-ab206419'>ab206419</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa whole cell lysate at 20 µg

Lane 2:

CD105  knockout HeLa whole cell lysate at 20 µg

Predicted band size: 70 kDa

false

• WB

Supplier Data

Western blot - Anti-CD105 antibody [EPR19911] - BSA and Azide free (AB251457)

This data was developed using ab206419, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1692830, 18223685, 11164421).

All lanes:

Western blot - Anti-CD105 antibody [EPR19911] (<a href='/en-us/products/primary-antibodies/cd105-antibody-epr19911-ab206419'>ab206419</a>) at 1/1000 dilution

Lane 1:

THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg

Lane 2:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lane 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 70 kDa

Observed band size: 90 kDa,95 kDa

false

Exposure time: 3min