AB221675

Anti-CD105 antibody [EPR21846]

RabMAb
Recombinant
20ul selling size
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(34 Publications)

Anti-CD105 antibody [EPR21846] (ab221675) is a rabbit monoclonal antibody detecting CD105 in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF. Suitable for Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

CD105, Edg, Eng, Endoglin, Cell surface MJ7/18 antigen

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution. Positive staining in the endothelial cells of blood vessels in mouse brain tissue section (PMID : 24699047). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse lung is observed (PMID : 14528280). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry - Anti-CD105 antibody [EPR21846] (AB221675)

Flow Cyt

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Flow Cytometry - Anti-CD105 antibody [EPR21846] (AB221675)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line (left panel) and bEND.3 (mouse brain endothelioma cell line) cell line (right panel) labeling CD105 with ab221675 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on total viable cells.

Negative control : NIH/3T3. (PMID : 8194490).

Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] (AB221675)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution (green). Positive staining in the hepatic sinusoidal endothelial cells on mouse liver tissue section (PMID : 12947156; 24507660). The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] (AB221675)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] (AB221675)

Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD105 with ab221675 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Neuro-2a cell line. Negative control : NIH/3T3 (PMID : 8194490).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse E14.5 liver is observed (PMID : 18805961). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-CD105 antibody [EPR21846] (AB221675)

CD105 was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab221675 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221675 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : bEND.3 whole cell lysate 10 μg (Input).

Lane 2 : ab221675 IP in bEND.3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab221675 in bEND.3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-CD105 antibody [EPR21846] (ab221675)

Predicted band size: 70 kDa

Observed band size: 95 kDa

false

Supplier Data

Western blot - Anti-CD105 antibody [EPR21846] (AB221675)

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular mass is consistent with the literature (PMID : 8932339). Negative control : NIH/3T3 (PMID : 8194490).

All lanes:

Western blot - Anti-CD105 antibody [EPR21846] (ab221675) at 1/1000 dilution

Lane 1:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Lane 3:

Mouse lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 70 kDa

Observed band size: 95 kDa

true

Exposure time: 70s

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405)
421 Alexa Fluor® 405

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
421 Alexa Fluor® 405

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
603 Alexa Fluor® 568

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)
702 Alexa Fluor® 680

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 680) preadsorbed

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21846

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ICC/IF, WB, IHC-Fr, IP, Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "Perform heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (<a href='/en-us/products/unavailable/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab94681'>ab94681</a>)." } } }

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Product details

What is this antibody validated in?
Anti-CD105 antibody [EPR21846] (ab221675) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of CD105?
Anti-CD105 [EPR21846] (ab221675) specifically detects a band for CD105 (UniProt: Q63961) at a molecular weight of 70kDa.

Trusted by the scientific community
Anti-CD105 [EPR21846] (ab221675) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR21846] also available for your convenience: ab221675, Carrier free - ab231832, Alexa Fluor® 488 - ab237388, Alexa Fluor® 647 - ab307528, PE - ab314279, FITC - ab314950

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed : 10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed : 28530658). May play a role in the binding of endothelial cells to integrins. Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).

See full target information Eng

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:281 PubMed40713623

2025

Dual targeting of VEGFR2 and CSF1R with SYHA1813 confers novel strategy for treating both BRAF wild-type and mutant melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Shi,Haotian Tang,Linjiang Tong,Peiran Song,Yuqing Huang,Zhipeng Wan,Gege Huang,Qiupei Liu,Zhengsheng Zhan,Yu Zhou,Yuantong Li,Jiaxin Wen,Bencan Tang,Wenhu Duan,Jian Ding,Xiaorui Li,Hua Xie

Molecular neurodegeneration 20:84 PubMed40702549

2025

Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pingao Zhang,Chenghuan Song,Jiyun Shi,Zijie Wei,Jing Wang,Wanying Huang,Rui Zhang,Jintao Wang,Xiaoli Yang,Gang Wang,Xiaoling Gao,Yongfang Zhang,Hongzhuan Chen,Hao Wang

Biochemistry and biophysics reports 43:102154 PubMed40697519

2025

An innovative compound that promotes oral wound healing via mobilizing gingival mesenchymal stem cell homing.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yang,Yue Song,Yao Jiao,Siyan Liu,Yi Liu,Lijia Guo,Yitong Liu

CNS neuroscience & therapeutics 31:e70463 PubMed40485217

2025

DeSUMOylation of IGF2BP2 Promotes Neuronal Differentiation of OM-MSCs by Stabilizing SOX11 to Ameliorate Brain Injury After Intracerebral Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jun He,Yuhan Luo,Chuang Wang,Chonghua Jiang,Jian Wang,Ying Xia

Cell biology and toxicology 41:85 PubMed40369223

2025

IGFBP3 enhances adipose-derived stem cell function in soft tissue injury repair via ITGB1 and ERK pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Sirui Tian,Haiyang Yu,Ruoxuan Yang,Heshi Wang,Baohong Zhao,Danning Wang

Reproductive medicine and biology 24:e12639 PubMed40110174

2025

The effect of ionizing radiation on testicular interstitial stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Esraa Tamam,Chen Yan,Reiko Sekiya,Tao-Sheng Li

NPJ Regenerative medicine 9:43 PubMed39738050

2024

Macrophage-derived extracellular vesicles transfer mitochondria to adipocytes and promote adipocyte-myofibroblast transition in epidural fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Hua,Jinpeng Sun,Mohan Shi,Rui Mei,Zeyuan Song,Jun Liu,Mingshun Zhang

Stem cell research & therapy 15:380 PubMed39456064

2024

Intrauterine adhesions repair with menstrual blood-derived mesenchymal stem cells via CXCL13-CXCR5 signal axis and its mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Luo,Xun Zeng,Li Luo

Cell death discovery 10:381 PubMed39187478

2024

Tert promotes cardiac regenerative repair after MI through alleviating ROS-induced DNA damage response in cardiomyocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Wei,Yilin Zhou,Enge Shao,Xiaoran Shi,Yuan Han,Yeshen Zhang,Guoquan Wei,Hao Zheng,Senlin Huang,Yanmei Chen,Jie Sun,Yulin Liao,Wangjun Liao,Yanbing Wang,Jianping Bin,Xinzhong Li

iScience 27:110467 PubMed39104411

2024

PIK3CA mutations enhance the adipogenesis of ADSCs in facial infiltrating lipomatosis through TRPV1.

Applications

Unspecified application

Species

Unspecified reactive species

Hongrui Chen,Bin Sun,Wei Gao,Yajing Qiu,Wei Wei,Yongguo Li,Wei Ye,Haoliang Song,Chen Hua,Xiaoxi Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD105 antibody [EPR21846]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Flow Cytometry - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] (AB221675)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

Immunoprecipitation - Anti-CD105 antibody [EPR21846] (AB221675)

Western blot - Anti-CD105 antibody [EPR21846] (AB221675)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

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Aliquoting information

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Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

Product promise