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Mouse Monoclonal CD105 antibody. Suitable for Flow Cyt, WB, sELISA, ICC/IF and reacts with Human samples. Cited in 29 publications.

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Images

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBsELISAICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1.00000-5.00000 µg/mL

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

For sandwich ELISA, use this antibody as Capture at 5 μg/ml with Rabbit polyclonal to CD105 (ab21224) as Detection.

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Target data

Function

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:8370410, PubMed:21737454, PubMed:22347366, PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).

Alternative names

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Mouse Monoclonal CD105 antibody. Suitable for Flow Cyt, WB, sELISA, ICC/IF and reacts with Human samples. Cited in 29 publications.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

MEM-226

Purification technique

Affinity purification Protein A

Concentration
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Purification notes

Purified from TCS. Purity >95% by SDS-PAGE.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

    Overlay histogram showing U937 (Human histiocytic lymphoma cell line) cells stained with ab2529 (red line).

    The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2529, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Western blot - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

    Lanes 1 - 3: Merged signal (red and green). Green - ab2529 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab2529 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab2529 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD105 antibody [MEM-226] (AB2529) at 1/1000 dilution

    Lane 1: Wild-type HeLa whole cell lysate at 20 µg

    Lane 2: CD105 knockout HeLa whole cell lysate at 20 µg

    Lane 3: HUVEC whole cell lysate at 20 µg

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)

    ICC/IF image of ab2529 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

    The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2529, 10 μg/ml) overnight at +4°C. The secondary antibody (green) was ab69879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM.

  • Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)

    Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.

  • Western blot - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

    All lanes: Western blot - Anti-CD105 antibody [MEM-226] (AB2529) at 5 µg/mL

    All lanes: Human colon tissue lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 70 kDa

    Observed band size: 45 kDa, 55 kDa, 80 kDa

  • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529), expandable thumbnail

    Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

    Flow cytometry analysis showing separation of HUVEC cells stained using ab2529 (concentration in sample 1.67 ?g/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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