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AB69772

Anti-CD105 antibody [MEM-229]

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(15 Publications)

Mouse Monoclonal CD105 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Pig samples. Cited in 15 publications. Immunogen corresponding to Recombinant Virus within Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (AB69772)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (AB69772)

ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (AB69772)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (AB69772)

Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5μg/mL. DAPI (blue) was used as nuclear counterstain.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-229

Isotype

IgG2a

Carrier free

No

Reacts with

Human, Pig

Applications

IHC-Fr, ICC/IF

applications

Immunogen

Recombinant Virus within Human ENG. The exact immunogen used to generate this antibody is proprietary information.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/200", "IHCFr-species-notes": "<p>Fix with Acetone.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:9370 PubMed38653980

2024

Derivation and long-term maintenance of porcine skeletal muscle progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Susan O Dan-Jumbo,Susanna E Riley,Yennifer Cortes-Araya,William Ho,Seungmee Lee,Thomas Thrower,Cristina L Esteves,F Xavier Donadeu

Frontiers in medicine 8:710079 PubMed34621759

2021

Reduced Expression of Erythropoietin After Intravitreal Ranibizumab in Proliferative Diabetic Retinopathy Patients-Retrospective Interventional Study.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Jing Feng,Yanhong Shi,Fuxiao Luan,Fang Ma,Yingjie Wang,Weiqiang Yang,Yong Tao

Cancer letters 515:86-95 PubMed34052329

2021

PERK-eIF2α-ERK1/2 axis drives mesenchymal-endothelial transition of cancer-associated fibroblasts in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenrun Cai,Xugang Sun,Fanjie Jin,Di Xiao,Hui Li,Huizhi Sun,Yifei Wang,Yang Lu,Jing Liu,Chongbiao Huang,Xiuchao Wang,Song Gao,Hongwei Wang,Chuntao Gao,Tiansuo Zhao,Jihui Hao

Cell and tissue research 384:367-387 PubMed33496880

2021

Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT).

Applications

Unspecified application

Species

Unspecified reactive species

Jianying Zhang,Feng Li,Kelly M Williamson,Susheng Tan,Devon Scott,Kentaro Onishi,MaCalus V Hogan,James H-C Wang

Scientific reports 10:21094 PubMed33273664

2020

Anti-IL-17A treatment reduces serum inflammatory, angiogenic and tissue remodeling biomarkers accompanied by less synovial high endothelial venules in peripheral spondyloarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Merlijn H Kaaij,Boy Helder,Leonieke J J van Mens,Marleen G H van de Sande,Dominique L P Baeten,Sander W Tas

Frontiers in bioengineering and biotechnology 7:155 PubMed31355191

2019

Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqiu Yang,Vanessa Marigo Rocha Pinto,Wei Duan,Erica E Paxton,Jenna H Dessauer,William Ryan,Mandi J Lopez

Stem cell research & therapy 10:164 PubMed31196185

2019

HLA-DR expression in clinical-grade bone marrow-derived multipotent mesenchymal stromal cells: a two-site study.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Grau-Vorster,Anita Laitinen,Johanna Nystedt,Joaquim Vives

Stem cell research & therapy 9:178 PubMed29973295

2018

Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Calle,Clara Barrajón-Masa,Ernesto Gómez-Fidalgo,Mercedes Martín-Lluch,Paloma Cruz-Vigo,Raúl Sánchez-Sánchez,Miguel Ángel Ramírez

Cellular physiology and biochemistry : internation 47:1785-1799 PubMed29953975

2018

Gremlin1 Delivered by Mesenchymal Stromal Cells Promoted Epithelial-Mesenchymal Transition in Human Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dongxi Hong,Te Liu,Weijun Huang,Yan Liao,Lin Wang,Zhen Zhang,Haixuan Chen,Xinchun Zhang,Qiuling Xiang

Journal of tissue engineering and regenerative medicine 12:e2001-e2009 PubMed29272079

2018

Development of lacrimal gland spheroids for lacrimal gland tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Isobel Massie,Kristina Spaniol,Andreas Barbian,Gerd Geerling,Marco Metzger,Stefan Schrader
View all publications
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