Mouse Monoclonal CD105 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Pig samples. Cited in 12 publications. Immunogen corresponding to Recombinant Virus within Human ENG.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (AB69772), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-mem-229-ab69772--immunocytochemistry-immunofluorescence-img101713.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (AB69772)")
![Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (AB69772), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-mem-229-ab69772--immunohistochemistry-frozen-sections-img63548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (AB69772)")
Publications
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- Frontiers in medicine 8:7100792021Reduced Expression of Erythropoietin After Intravitreal Ranibizumab in Proliferative Diabetic Retinopathy Patients-Retrospective Interventional Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Chen,Jing Feng,Yanhong Shi,Fuxiao Luan,Fang Ma,Yingjie Wang,Weiqiang Yang,Yong TaoPubMed 34621759
- Cancer letters 515:86-952021PERK-eIF2α-ERK1/2 axis drives mesenchymal-endothelial transition of cancer-associated fibroblasts in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenrun Cai,Xugang Sun,Fanjie Jin,Di Xiao,Hui Li,Huizhi Sun,Yifei Wang,Yang Lu,Jing Liu,Chongbiao Huang,Xiuchao Wang,Song Gao,Hongwei Wang,Chuntao Gao,Tiansuo Zhao,Jihui HaoPubMed 34052329
- Scientific reports 10:210942020Anti-IL-17A treatment reduces serum inflammatory, angiogenic and tissue remodeling biomarkers accompanied by less synovial high endothelial venules in peripheral spondyloarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMerlijn H Kaaij,Boy Helder,Leonieke J J van Mens,Marleen G H van de Sande,Dominique L P Baeten,Sander W TasPubMed 33273664
- Frontiers in bioengineering and biotechnology 7:1552019Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingqiu Yang et. al.PubMed 31355191
- Stem cell research & therapy 10:1642019HLA-DR expression in clinical-grade bone marrow-derived multipotent mesenchymal stromal cells: a two-site study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarta Grau-Vorster et. al.PubMed 31196185
- Stem cell research & therapy 9:1782018Iberian pig mesenchymal stem/stromal cells from dermal skin, abdominal and subcutaneous adipose tissues, and peripheral blood: in vitro characterization and migratory properties in inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Calle et. al.PubMed 29973295
- Cellular physiology and biochemistry : internation 47:1785-17992018Gremlin1 Delivered by Mesenchymal Stromal Cells Promoted Epithelial-Mesenchymal Transition in Human Esophageal Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongxi Hong et. al.PubMed 29953975
- Journal of tissue engineering and regenerative medicine 12:e2001-e20092018Development of lacrimal gland spheroids for lacrimal gland tissue regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsobel Massie,Kristina Spaniol,Andreas Barbian,Gerd Geerling,Marco Metzger,Stefan SchraderPubMed 29272079
- Regenerative therapy 7:24-332017Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYasuhiro Maruya et. al.PubMed 30271849
- Regenerative therapy 6:83-892017Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNobuyuki Kaibuchi et. al.PubMed 30271842
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Virus within Human ENG. The exact immunogen used to generate this antibody is proprietary information. Database link P17813
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IHC-Fr | |
|---|---|---|
| Human | Tested | Expected |
| Pig | Expected | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 1/200 | Notes Fix with Acetone. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:21737454, PubMed:23300529). Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed:1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529, PubMed:8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed:21737454, PubMed:22347366, PubMed:23300529).
Alternative names
CD105, END, ENG, Endoglin
Recommended products
Mouse Monoclonal CD105 antibody. Suitable for ICC/IF, IHC-Fr and reacts with Human, Pig samples. Cited in 12 publications. Immunogen corresponding to Recombinant Virus within Human ENG.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Virus within Human ENG. The exact immunogen used to generate this antibody is proprietary information. Database link P17813
- Clone number
- MEM-229
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
- Biological function summary
Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.
- Pathways
CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.
- Associated diseases and disorders
CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-mem-229-ab69772--immunocytochemistry-immunofluorescence-img101713.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)")
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-229] (ab69772)
ab69772 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69772 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
![Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772), expandable thumbnail](https://content.abcam.com/products/images/cd105-antibody-mem-229-ab69772--immunohistochemistry-frozen-sections-img63548.jpg "Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)")
Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [MEM-229] (ab69772)
Immunohistochemical analysis of frozen infarcted porcine heart labelling CD105 (green) with ab69772 at 5μg/mL. DAPI (blue) was used as nuclear counterstain.
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