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AB156756

Anti-CD105 antibody [OTI8A1]

2

(2 Reviews)

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(25 Publications)

Mouse Monoclonal CD105 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human, Mouse, African green monkey, Dog, Rat samples. Cited in 25 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

4 Images
Flow Cytometry (Intracellular) - Anti-CD105 antibody [OTI8A1] (AB156756)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD105 antibody [OTI8A1] (AB156756)

Overlay histogram showing U937 cells stained with ab156756 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab156756, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [OTI8A1] (AB156756)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [OTI8A1] (AB156756)

Immunofluorescent analysis of HeLa cells labeling CHN1 with ab156756 at 1/100.

Western blot - Anti-CD105 antibody [OTI8A1] (AB156756)
  • WB

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Western blot - Anti-CD105 antibody [OTI8A1] (AB156756)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211854)

All lanes:

Western blot - Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY CD105 at 5 µg

Predicted band size: 70 kDa

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Western blot - Anti-CD105 antibody [OTI8A1] (AB156756)
  • WB

Supplier Data

Western blot - Anti-CD105 antibody [OTI8A1] (AB156756)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211696)

All lanes:

Western blot - Anti-CD105 antibody [OTI8A1] (ab156756) at 1/200 dilution

Lane 1:

HepG2 cell lysate at 35 µg

Lane 2:

HeLa cell lysate at 35 µg

Lane 3:

SVT2 cell lysate at 35 µg

Lane 4:

A549 cell lysate at 35 µg

Lane 5:

COS7 cell lysate at 35 µg

Lane 6:

Jurkat cell lysate at 35 µg

Lane 7:

MDCK cell lysate at 35 µg

Lane 8:

PC12 cell lysate at 35 µg

Lane 9:

MCF7 cell lysate at 35 µg

Predicted band size: 70 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI8A1

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ENG.

P17813

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/4000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

The clone number has been updated from 8A1 to OTI8A1, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab156756 is purified from TCS by affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:84 PubMed40702549

2025

Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pingao Zhang,Chenghuan Song,Jiyun Shi,Zijie Wei,Jing Wang,Wanying Huang,Rui Zhang,Jintao Wang,Xiaoli Yang,Gang Wang,Xiaoling Gao,Yongfang Zhang,Hongzhuan Chen,Hao Wang

Pharmaceutics 16: PubMed39458645

2024

Priming Mesenchymal Stem Cells with Lipopolysaccharide Boosts the Immunomodulatory and Regenerative Activity of Secreted Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Aina Areny-Balagueró,Marta Camprubí-Rimblas,Elena Campaña-Duel,Anna Solé-Porta,Adrián Ceccato,Anna Roig,John G Laffey,Daniel Closa,Antonio Artigas

Bioengineering (Basel, Switzerland) 11: PubMed38927821

2024

Development of Subcutaneous SSEA3- or SSEA4-Positive Cell Capture Device.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhide Nakayama,Ryosuke Iwai

Cureus 16:e60682 PubMed38899254

2024

Brain Vascular Expression of Monomeric C-Reactive Protein Is Blocked by C10M Following Intraperitoneal Injection in an ApoE-/- Murine Model of Dyslipidemia: An Immunohistochemical Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ylenia Pastorello,Aurelio Pio Russo,Claudia Bănescu,Vittorio Caprio,Zsolt Gáll,Lawrence Potempa,Bogdan Cordoș,Mario Di Napoli,Mark Slevin

Nature communications 14:6148 PubMed37783697

2023

Mechanically induced pyroptosis enhances cardiosphere oxidative stress resistance and metabolism for myocardial infarction therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yingwei Wang,Qi Li,Jupeng Zhao,Jiamin Chen,Dongxue Wu,Youling Zheng,Jiaxin Wu,Jie Liu,Jianlong Lu,Jianhua Zhang,Zheng Wu

International journal of molecular sciences 24: PubMed37108673

2023

Impact of Adipose Tissue Depot Harvesting Site on the Multilineage Induction Capacity of Male Rat Adipose-Derived Mesenchymal Stem Cells: An In Vitro Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hussein M El-Husseiny,Masahiro Kaneda,Eman A Mady,Tadashi Yoshida,Ahmed S Doghish,Ryou Tanaka

Stem cell research & therapy 14:89 PubMed37061739

2023

Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Sahoo,Mukesh Damala,Jilu Jaffet,Deeksha Prasad,Sayan Basu,Vivek Singh

Stem cell research & therapy 13:343 PubMed35883119

2022

Neurotrophic factor-secreting cells restored endogenous hippocampal neurogenesis through the Wnt/β-catenin signaling pathway in AD model mice.

Applications

Unspecified application

Species

Unspecified reactive species

Gozal Bahlakeh,Reza Rahbarghazi,Ali Abedelahi,Saeed Sadigh-Eteghad,Mohammad Karimipour

Biomedicines 9: PubMed33671438

2021

Platelet-Derived Growth Factor Stimulated Migration of Bone Marrow Mesenchymal Stem Cells into an Injectable Gelatin-Hydroxyphenyl Propionic Acid Matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Niu,Teck Chuan Lim,Abdulmonem Alshihri,Ravikumar Rajappa,Lishan Wang,Motoichi Kurisawa,Myron Spector

Biomedical papers of the Medical Faculty of the Un : PubMed33325456

2020

Compare features of human dental pulp cells cultured from pulp tissues with and without cryopreservation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Yan,Ola A Nada,Ralf Smeets,Martin Gosau,Reinhard E Friedrich,Lan Kluwe
View all publications
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