Mouse Monoclonal CD109 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD109 protein.
Images
![Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (AB47169), expandable thumbnail](https://content.abcam.com/products/images/cd109-antibody-b-e47-bsa-and-azide-free-ab47169--flow-cytometry-img58082.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (AB47169)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing CD109 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6YHK3
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Modulates negatively TGFB1 signaling in keratinocytes.
Alternative names
CD109, CPAMD7, CD109 antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150
Recommended products
Mouse Monoclonal CD109 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD109 protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3 - 7.5
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing CD109 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q6YHK3
- Carrier free
- Yes
- Clone number
- B-E47
- Purification technique
- Affinity purification
- Specificity
Recognises a 170 kDa protein.
- Light chain type
- kappa
- Concentration
- Purification notes
Purified by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C, -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 4th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD109 also known as platelet activation antigen 3 is a glycoprotein with a molecular weight around 170 kDa. You can find it mostly expressed on the surface of platelets activated T-cells epithelial cells and some tumor cells. This protein belongs to the α2-macroglobulin/C3 C4 C5 family and plays a part in regulating several cellular activities. CD109 acts mechanically as a glycosylphosphatidylinositol (GPI)-anchored protein which suggests its involvement in signal transduction processes.
- Biological function summary
The protein CD109 modulates several cellular functions including cell proliferation differentiation and immune response regulation. The protein works as a negative regulator of TGF-β signaling by forming a complex with the TGF-β receptor. This activity influences processes like wound healing and immune regulation where TGF-β plays a vital role. CD109 expression also impacts cellular interactions and behavior in the tumor microenvironment adding additional layers of regulation to its function.
- Pathways
CD109 participates in the TGF-β signaling pathway and has effects on processes like fibrosis and epithelial-mesenchymal transition (EMT). This involvement connects CD109 functionally to components such as Smad proteins which mediate the signal for TGF-β pathway downstream effects. CD109's modulation within this pathway suggests a regulatory influence that may alter cellular responses under different physiological conditions.
- Associated diseases and disorders
Research associates CD109 with various cancers where it appears to influence tumor progression and metastasis. The protein shows altered expression in glioblastoma and certain leukemias. Its involvement in these cancers links CD109 to other cancer-related proteins such as epidermal growth factor receptor (EGFR) which often shows interplay in signaling pathways involved in cell proliferation and survival. Understanding CD109's function and impact can offer insights into new therapeutic targets in oncology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (ab47169), expandable thumbnail](https://content.abcam.com/products/images/cd109-antibody-b-e47-bsa-and-azide-free-ab47169--flow-cytometry-img58082.jpg "Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (ab47169)")
Flow Cytometry - Anti-CD109 antibody [B-E47] - BSA and Azide free (ab47169)
A typical staining pattern with ab47169 of Eahy 926 cell line.
This image was generated using the ascites version of the product.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com