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Rabbit Recombinant Monoclonal CD109 antibody. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (AB318142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (AB318142), expandable thumbnail
  • Western blot - Anti-CD109 antibody [EPR29008-59] (AB318142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (AB318142), expandable thumbnail
  • Western blot - Anti-CD109 antibody [EPR29008-59] (AB318142), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow CytIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/5000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Modulates negatively TGFB1 signaling in keratinocytes.

Alternative names

CD109 antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150, CPAMD7, CD109

Recommended products

Rabbit Recombinant Monoclonal CD109 antibody. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

CD109 antigen, 150 kDa TGF-beta-1-binding protein, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7, Platelet-specific Gov antigen, p180, r150, CPAMD7, CD109

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR29008-59

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD109 also known as platelet activation antigen 3 is a glycoprotein with a molecular weight around 170 kDa. You can find it mostly expressed on the surface of platelets activated T-cells epithelial cells and some tumor cells. This protein belongs to the α2-macroglobulin/C3 C4 C5 family and plays a part in regulating several cellular activities. CD109 acts mechanically as a glycosylphosphatidylinositol (GPI)-anchored protein which suggests its involvement in signal transduction processes.

Biological function summary

The protein CD109 modulates several cellular functions including cell proliferation differentiation and immune response regulation. The protein works as a negative regulator of TGF-β signaling by forming a complex with the TGF-β receptor. This activity influences processes like wound healing and immune regulation where TGF-β plays a vital role. CD109 expression also impacts cellular interactions and behavior in the tumor microenvironment adding additional layers of regulation to its function.

Pathways

CD109 participates in the TGF-β signaling pathway and has effects on processes like fibrosis and epithelial-mesenchymal transition (EMT). This involvement connects CD109 functionally to components such as Smad proteins which mediate the signal for TGF-β pathway downstream effects. CD109's modulation within this pathway suggests a regulatory influence that may alter cellular responses under different physiological conditions.

Associated diseases and disorders

Research associates CD109 with various cancers where it appears to influence tumor progression and metastasis. The protein shows altered expression in glioblastoma and certain leukemias. Its involvement in these cancers links CD109 to other cancer-related proteins such as epidermal growth factor receptor (EGFR) which often shows interplay in signaling pathways involved in cell proliferation and survival. Understanding CD109's function and impact can offer insights into new therapeutic targets in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CD109 with ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels in human colon. The section was incubated with ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling CD109 with ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels in human lung. The section was incubated with ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142)

    Negative control: SK-BR-3(PMID: 20034764)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD109 with ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cervical carcinoma. The section was incubated with ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142)

    The band at ~120KDa may represent a cleaved N-terminal region of CD109 (PMID: 33738281)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD109 antibody [EPR29008-59] (ab318142) at 1/1000 dilution

    Lane 1: Human ovary cancer tissue lysate at 20 µg

    Lane 2: Human liver cancer tissue lysate at 20 µg

    Lane 3: Human pancreas cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] (ab318142)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling CD109 with ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels of human lung carcinoma. The section was incubated with ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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