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Rabbit Recombinant Monoclonal CD109 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (AB318143), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (AB318143), expandable thumbnail
  • Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (AB318143), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (AB318143), expandable thumbnail
  • Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (AB318143), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow CytIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Modulates negatively TGFB1 signaling in keratinocytes.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD109 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29008-59
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab318143 is the carrier-free version of Anti-CD109 antibody [EPR29008-59] ab318142.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD109 also known as platelet activation antigen 3 is a glycoprotein with a molecular weight around 170 kDa. You can find it mostly expressed on the surface of platelets activated T-cells epithelial cells and some tumor cells. This protein belongs to the α2-macroglobulin/C3 C4 C5 family and plays a part in regulating several cellular activities. CD109 acts mechanically as a glycosylphosphatidylinositol (GPI)-anchored protein which suggests its involvement in signal transduction processes.

Biological function summary

The protein CD109 modulates several cellular functions including cell proliferation differentiation and immune response regulation. The protein works as a negative regulator of TGF-β signaling by forming a complex with the TGF-β receptor. This activity influences processes like wound healing and immune regulation where TGF-β plays a vital role. CD109 expression also impacts cellular interactions and behavior in the tumor microenvironment adding additional layers of regulation to its function.

Pathways

CD109 participates in the TGF-β signaling pathway and has effects on processes like fibrosis and epithelial-mesenchymal transition (EMT). This involvement connects CD109 functionally to components such as Smad proteins which mediate the signal for TGF-β pathway downstream effects. CD109's modulation within this pathway suggests a regulatory influence that may alter cellular responses under different physiological conditions.

Associated diseases and disorders

Research associates CD109 with various cancers where it appears to influence tumor progression and metastasis. The protein shows altered expression in glioblastoma and certain leukemias. Its involvement in these cancers links CD109 to other cancer-related proteins such as epidermal growth factor receptor (EGFR) which often shows interplay in signaling pathways involved in cell proliferation and survival. Understanding CD109's function and impact can offer insights into new therapeutic targets in oncology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CD109 with Anti-CD109 antibody [EPR29008-59] ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels in human colon. The section was incubated with Anti-CD109 antibody [EPR29008-59] ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling CD109 with Anti-CD109 antibody [EPR29008-59] ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels in human lung. The section was incubated with Anti-CD109 antibody [EPR29008-59] ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    Negative control: SK-BR-3(PMID: 20034764)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD109 antibody [EPR29008-59] (Anti-CD109 antibody [EPR29008-59] ab318142) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD109 with Anti-CD109 antibody [EPR29008-59] ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cervical carcinoma. The section was incubated with Anti-CD109 antibody [EPR29008-59] ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Western blot - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    The band at ~120KDa may represent a cleaved N-terminal region of CD109 (PMID: 33738281)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-CD109 antibody [EPR29008-59] (Anti-CD109 antibody [EPR29008-59] ab318142) at 1/1000 dilution

    Lane 1: Human ovary cancer tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Human liver cancer tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Human pancreas cancer tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 200 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD109 antibody [EPR29008-59] - BSA and Azide free (ab318143)

    This data was developed using Anti-CD109 antibody [EPR29008-59] ab318142, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling CD109 with Anti-CD109 antibody [EPR29008-59] ab318142 at 1/5000 (0.1 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on blood vessels of human lung carcinoma. The section was incubated with Anti-CD109 antibody [EPR29008-59] ab318142 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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