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AB280956

Anti-CD116 antibody [EPR24554-26] - BSA and Azide free

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Rabbit Recombinant Monoclonal CD116 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD116, Csfgmra, Csf2ra, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha

2 Images
Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (AB280956)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (AB280956)

This data was developed using ab280951, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse blood cells cells labelling CD116 with ab280951 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Expression of GMRa is restrcted to cells of the myeloid, but not lymphoid (PMID 17694571).Gated on viable cells.

Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (AB280956)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD116 antibody [EPR24554-26] - BSA and Azide free (AB280956)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab280951).

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab280951 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab280951 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 μl at 1.0 μg/ml (1/1990)) for 30min on ice. The cells were simultaneously stained with CD11b.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

  • Unconjugated

    Anti-CD116 antibody [EPR24554-26]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD116 antibody [EPR24554-26]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD116 antibody [EPR24554-26]

  • 578 PE

    PE Anti-CD116 antibody [EPR24554-26]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24554-26

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

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Product details

Please Note: Clone [EPR24554-26] (this product) is different to that of [EPR24554-113] (ab280357) for the same target.

ab280956 is the carrier-free version of ab280951.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).
Biological function summary

The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.

Pathways

CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.

Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.. Isoform 2. Contrary to isoform 1, does not trigger cell proliferation in response to GM-CSF.. Isoform 3. Contrary to isoform 1, does not trigger cell proliferation in response to GM-CSF.
See full target information Csf2ra
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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