Rabbit Recombinant Monoclonal CD116 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--western-blot-img408793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408795.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408794.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408796.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (AB308295)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
Alternative names
CD116, CSF2R, CSF2RY, CSF2RA, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116
Recommended products
Rabbit Recombinant Monoclonal CD116 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26505-444
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).
- Biological function summary
The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.
- Pathways
CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.
- Associated diseases and disorders
Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--western-blot-img408793.jpg "Western blot - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)")
Western blot - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
Low expression: HT-29(PMID:28811578), MCF7, human colon.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.
The higher molecular weight observed is due to the high glycosylation of this receptor.
All lanes: Western blot - Anti-CD116 antibody [EPR26505-444] (Anti-CD116 antibody [EPR26505-444] ab308294) at 1/1000 dilution
Lane 1: SK-MEL-2 whole cell lysate at 20 µg
Lane 2: HT-29 whole cell lysate at 20 µg
Lane 3: MCF7 whole cell lysate at 20 µg
Lane 4: Human placenta tissue lysate at 20 µg
Lane 5: Human lymphoma tissue lysate at 20 µg
Lane 6: Human colon tissue lysate at 20 µg
SecondaryLanes 1 - 6: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Lanes 1 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 80 kDa
Exposure time: 92s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408795.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)
This data was produced using Anti-CD116 antibody [EPR26505-444] ab308294, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling CD116 with Anti-CD116 antibody [EPR26505-444] ab308294 at 1/100 dilution (5.04 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human diffuse large B-cell lymphoma.
The section was incubated with Anti-CD116 antibody [EPR26505-444] ab308294 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408794.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)
This data was produced using Anti-CD116 antibody [EPR26505-444] ab308294, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD116 with Anti-CD116 antibody [EPR26505-444] ab308294 at 1/100 dilution (5.04 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human tonsil.
The section was incubated with Anti-CD116 antibody [EPR26505-444] ab308294 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295), expandable thumbnail](https://content.abcam.com/products/images/cd116-antibody-epr26505-444-bsa-and-azide-free-ab308295--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img408796.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD116 antibody [EPR26505-444] - BSA and Azide free (ab308295)
This data was produced using Anti-CD116 antibody [EPR26505-444] ab308294, the same clone but in a different formulation.
Immunohistochemical analysis of paraffin-embedded cell pellets labeling CD116 with Anti-CD116 antibody [EPR26505-444] ab308294 at 1/100 dilution (5.04 ug/ml) followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on SK-MEL-2 (image A), no staining on MCF7 (image B) and HT-29 (image C).
The section was incubated with Anti-CD116 antibody [EPR26505-444] ab308294 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com