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AB260001

Anti-CD11a antibody [EPR22578-312] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ITAL antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD11a, Lfa-1, Ly-15, Itgal, Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, Lymphocyte antigen 15, LFA-1A

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the mouse spleen (PMID : 2185332). The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228964).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the immune cells in the mouse small intestine. The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228964).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] - BSA and Azide free (AB260001)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the immune cells in the mouse colon (PMID : 12434422). The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228964).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22578-312

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab260001 is the carrier-free version of ab228964.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11a also known as ITGAL and LFA-1 alpha chain or integrin alpha L is a protein that functions as an important component of the immune system. It has a molecular mass of approximately 180 kDa. CD11a is expressed predominantly in leukocytes enabling these immune cells to adhere and interact with other cells. This adhesive property is especially critical in the trafficking of leukocytes to sites of inflammation and infection. CD11a pairs with CD18 to form the leukocyte function-associated antigen-1 (LFA-1) complex which plays a significant role in cell signaling and immune responses.
Biological function summary

This protein enables leukocyte adhesion and transendothelial migration which are essential for the immune surveillance process. CD11a is a part of the integrin family known for regulating cell adhesion and interactions with the extracellular matrix. By forming a heterodimer with CD18 it mediates tight adhesion between immune cells and endothelial cells. This interaction aids in the immune response by enhancing the activation and movement of T cells and other leukocytes through the vascular endothelium into tissues.

Pathways

The protein participates in the immune cell trafficking pathway and the integrin signaling pathway. In these pathways CD11a contributes to the modulation of immune responses and cell movement. The interaction of CD11a with intercellular adhesion molecule 1 (ICAM-1) and other proteins within these pathways emphasizes its role in the firm adhesion of leukocytes during inflammation and infection ultimately facilitating leukocyte extravasation and tissue infiltration.

CD11a is highly relevant to autoimmune diseases such as psoriasis and multiple sclerosis. For instance in psoriasis the overactive immune response involves overexpression of integrins including CD11a leading to inflammation. The protein Efalizumab a monoclonal antibody against CD11a has been used in the treatment of psoriasis to reduce leukocyte adhesion and activation. Such relationships highlight CD11a's critical role in both normal immune function and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed : 2051027). Integrin ITGAL/ITGB2 is a receptor for F11R (By similarity). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 16234355, PubMed : 24158516). Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (By similarity). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed : 25108025). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
See full target information Itgal
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 12:395-425 PubMed33676046

2021

Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Yuan Mao,Yi-Pin Lv,Chuan-Jie Hao,Yong-Sheng Teng,Yu-Gang Liu,Ping Cheng,Shi-Ming Yang,Weisan Chen,Tao Liu,Quan-Ming Zou,Rui Xie,Jing-Yu Xu,Yuan Zhuang
View all publications
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Product promise

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