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AB119878

Anti-CD11a antibody [M17/4]

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(4 Publications)

Rat Monoclonal ITAL antibody. Suitable for IP, Flow Cyt, IHC-Fr and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing Itgal protein.

View Alternative Names

CD11a, Lfa-1, Ly-15, Itgal, Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, Lymphocyte antigen 15, LFA-1A

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M17/4

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

IP, IHC-Fr, Flow Cyt

applications

Immunogen

Cell preparation containing Itgal protein. The exact immunogen used to generate this antibody is proprietary information.

P24063

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>Application note: acetone fixation.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11a also known as ITGAL and LFA-1 alpha chain or integrin alpha L is a protein that functions as an important component of the immune system. It has a molecular mass of approximately 180 kDa. CD11a is expressed predominantly in leukocytes enabling these immune cells to adhere and interact with other cells. This adhesive property is especially critical in the trafficking of leukocytes to sites of inflammation and infection. CD11a pairs with CD18 to form the leukocyte function-associated antigen-1 (LFA-1) complex which plays a significant role in cell signaling and immune responses.
Biological function summary

This protein enables leukocyte adhesion and transendothelial migration which are essential for the immune surveillance process. CD11a is a part of the integrin family known for regulating cell adhesion and interactions with the extracellular matrix. By forming a heterodimer with CD18 it mediates tight adhesion between immune cells and endothelial cells. This interaction aids in the immune response by enhancing the activation and movement of T cells and other leukocytes through the vascular endothelium into tissues.

Pathways

The protein participates in the immune cell trafficking pathway and the integrin signaling pathway. In these pathways CD11a contributes to the modulation of immune responses and cell movement. The interaction of CD11a with intercellular adhesion molecule 1 (ICAM-1) and other proteins within these pathways emphasizes its role in the firm adhesion of leukocytes during inflammation and infection ultimately facilitating leukocyte extravasation and tissue infiltration.

CD11a is highly relevant to autoimmune diseases such as psoriasis and multiple sclerosis. For instance in psoriasis the overactive immune response involves overexpression of integrins including CD11a leading to inflammation. The protein Efalizumab a monoclonal antibody against CD11a has been used in the treatment of psoriasis to reduce leukocyte adhesion and activation. Such relationships highlight CD11a's critical role in both normal immune function and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed : 2051027). Integrin ITGAL/ITGB2 is a receptor for F11R (By similarity). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 16234355, PubMed : 24158516). Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (By similarity). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed : 25108025). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
See full target information Itgal
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 176:3356-65 PubMed16517703

2006

Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Georgi S Angelov,Philippe Guillaume,Marek Cebecauer,Giovanna Bosshard,Danijel Dojcinovic,Petra Baumgaertner,Immanuel F Luescher

Respiratory research 6:70 PubMed16011799

2005

The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Marc Beyer,Hongwei Wang,Nina Peters,Sandra Doths,Cordula Koerner-Rettberg,Peter J M Openshaw,Jürgen Schwarze

The Journal of experimental medicine 196:1515-21 PubMed12461086

2002

Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Geoffrey John Bellingan,Ping Xu,Helen Cooksley,Helen Cauldwell,Anthony Shock,Stephen Bottoms,Christopher Haslett,Steven Eugene Mutsaers,Geoffrey John Laurent

Blood 97:1282-8 PubMed11222371

2001

Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

T Papayannopoulou,G V Priestley,B Nakamoto,V Zafiropoulos,L M Scott,J M Harlan
View all publications
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