AB62817

Anti-CD11b antibody

Name: Anti-CD11b antibody (ab62817) | Abcam
Brand: Abcam Limited
SKU: AB62817
Price: 400 USD
Availability: InStock
Rating: 2 (8 reviews)

Lab Essentials
What is this?

(8 Reviews)

(15 Publications)

Goat Polyclonal CD11b antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ITGAM aa 800-850.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody (AB62817)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody (AB62817)

Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human ITGAM aa 800-850. The exact immunogen used to generate this antibody is proprietary information.

P11215

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab62817 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.

Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

See full target information ITGAM

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Geburtshilfe und Frauenheilkunde 85:710-723 PubMed40599898

2025

The Role of Microcirculatory Dysfunction During Paclitaxel Treatment as a Critical Co-Factor for the Development of Chemotherapy-Induced Peripheral Neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Reuter,Rika Bajorat,Fabian Müller-Graf,Amelie R Zitzmann,Volkmar Müller,Anna-Lena Pickhardt,Daniel A Reuter,Stephan H Böhm,Brigitte Vollmar

Nature nanotechnology 19:688-697 PubMed38225357

2024

Liquid-metal-based three-dimensional microelectrode arrays integrated with implantable ultrathin retinal prosthesis for vision restoration.

Applications

Unspecified application

Species

Unspecified reactive species

Won Gi Chung,Jiuk Jang,Gang Cui,Sanghoon Lee,Han Jeong,Haisu Kang,Hunkyu Seo,Sumin Kim,Enji Kim,Junwon Lee,Seung Geol Lee,Suk Ho Byeon,Jang-Ung Park

Frontiers in immunology 13:1096813 PubMed36713365

2023

β-hydroxybutyrate enhances bovine neutrophil adhesion by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyuan He,Kexin Wang,Mingchao Liu,Wen Zeng,Dong Li,Zolzaya Majigsuren,Tugsjargal Batbaatar,Yunfei Li,Siyuan Liu,Xiliang Du,Lin Lei,Yuxiang Song,Guowen Liu

Frontiers in psychiatry 12:803394 PubMed35211038

2022

Cell-Type Specific Deletion of CB2 Cannabinoid Receptors in Dopamine Neurons Induced Hyperactivity Phenotype: Possible Relevance to Attention-Deficit Hyperactivity Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Canseco-Alba,Branden Sanabria,Mariam Hammouda,Rollanda Bernadin,Marizel Mina,Qing-Rong Liu,Emmanuel S Onaivi

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

Cell 179:1609-1622.e16 PubMed31835035

2019

Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program.

Applications

Unspecified application

Species

Unspecified reactive species

Laufey Geirsdottir,Eyal David,Hadas Keren-Shaul,Assaf Weiner,Stefan Cornelius Bohlen,Jana Neuber,Adam Balic,Amir Giladi,Fadi Sheban,Charles-Antoine Dutertre,Christine Pfeifle,Francesca Peri,Antonella Raffo-Romero,Jacopo Vizioli,Kaspar Matiasek,Christian Scheiwe,Stephan Meckel,Kerstin Mätz-Rensing,Franziska van der Meer,Finnbogi Rutur Thormodsson,Christine Stadelmann,Noga Zilkha,Tali Kimchi,Florent Ginhoux,Igor Ulitsky,Daniel Erny,Ido Amit,Marco Prinz

American journal of physiology. Gastrointestinal a 317:G625-G639 PubMed31545917

2019

Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Arseniy E Yuzhalin,Su Yin Lim,Alex N Gordon-Weeks,Roman Fischer,Benedikt M Kessler,Dihua Yu,Ruth J Muschel

Oncotarget 10:3559-3575 PubMed31191826

2019

Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasha H Dosh,Nicola Jordan-Mahy,Christopher Sammon,Christine Le Maitre

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 16:231-243 PubMed30225791

2018

Evaluation of 3K3A-Activated Protein C to Treat Neonatal Hypoxic Ischemic Brain Injury in the Spiny Mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Stacey J Ellery,Madeleine G Goss,Nadine Brew,Hayley Dickinson,Nadia Hale,Domenic A LaRosa,David W Walker,Flora Y Wong

Journal of veterinary internal medicine 32:1692-1702 PubMed30084202

2018

Relationship of the mucosal microbiota to gastrointestinal inflammation and small cell intestinal lymphoma in cats.

Applications

Unspecified application

Species

Unspecified reactive species

Kayode Garraway,Chad M Johannes,Angela Bryan,John Peauroi,Giacomo Rossi,Min Zhang,Chong Wang,Karin Allenspach,Albert E Jergens

View all publications

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