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AB212505

Anti-CD11b antibody [C11b/660] - BSA and Azide free

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(3 Publications)

Mouse Monoclonal CD11b antibody. Carrier free. Suitable for Flow Cyt, FuncS, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ITGAM.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

C11b/660

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, FuncS

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ITGAM.

P11215

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Alternative Products

Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
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Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

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Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

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Primary Antibodies

AB52478

Anti-CD11b antibody [EP1345Y] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478)

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Primary Antibodies

AB187537

Anti-CD11b antibody [EP1345Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - BSA and Azide free (AB187537)

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Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

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Complementary Products

Assay Kits

AB277412

Human CD11b ELISA Kit

ELISA - Human CD11b ELISA Kit (AB277412)

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Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

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Primary Antibodies

AB183322

Anti-CD14 antibody [SP192]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Advanced Validation

Flow Cytometry - Anti-CD14 antibody [SP192] (AB183322)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

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  • 17
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>in 0.1ml volume.</p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab212505 is purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht) 46:1351-1368 PubMed37079186

2023

Dissecting the single-cell transcriptome network of macrophage and identifies a signature to predict prognosis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyang Hu,Xing Jin,Weifeng Hong,Qihai Sui,Mengnan Zhao,Yiwei Huang,Ming Li,Qun Wang,Cheng Zhan,Zhencong Chen

Nature neuroscience 22:374-385 PubMed30718903

2019

Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning.

Applications

Unspecified application

Species

Unspecified reactive species

Carl M Sellgren,Jessica Gracias,Bradley Watmuff,Jonathan D Biag,Jessica M Thanos,Paul B Whittredge,Ting Fu,Kathleen Worringer,Hannah E Brown,Jennifer Wang,Ajamete Kaykas,Rakesh Karmacharya,Carleton P Goold,Steven D Sheridan,Roy H Perlis

Oncotarget 9:3690-3703 PubMed29423076

2018

Androgen receptor/let-7a signaling regulates breast tumor-initiating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Xiaozhen Liu,Shan Liu,Ying Qin,Xiaoqi Tian,Fengting Niu,Han Liu,Ning Liu,Yun Niu
View all publications
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Product promise

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