Name: Anti-CD11b antibody [EP1345Y] - C-terminal
SKU: ab52478
Rating: 5 (5 reviews)

Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) is a rabbit monoclonal antibody that is used to detect CD11b in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 70 publications

-Over 70 publications

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	IHC-FoFr	ICC/IF
Human	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes For unpurified use at 1 - 5 μg/ml Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes For unpurified use at 1/80

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/20000 - 1/50000

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

See full target information ITGAM

Function

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

Alternative names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1345Y
Purification technique: Affinity purification Protein A
Specificity: Testing of mouse and rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of CD11b?

Anti-CD11b [EP1345Y] - C-terminal (ab52478) specifically detects a band for CD11b (UniProt: P11215) at a molecular weight of 128kDa.

Trusted by the scientific community

Anti-CD11b [EP1345Y] - C-terminal (ab52478) was first used in a scientific publication in 2007 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists

Anti-CD11b [EP1345Y] - C-terminal (ab52478) has over 5 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EP1345Y] also available for your convenience:
ab52478, Carrier free - ab187537, PE - ab213186, APC - ab221248, Oligonucleotide - ab284435

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.

Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

Associated diseases and disorders

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Image from Sindhu et al PLoS One. 2015 Jul 22;10(7):e0133494. doi: 10.1371/journal.pone.0133494. eCollection 2015. Fig 5.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Enhanced expression of monocytes/ macrophage markers in the obese adipose tissue.
The protein expression (intensity) of monocyte/ macrophage markers was detected by immunohistochemistry (IHC) in the adipose tissue samples from lean, overweight, and obese individuals, 10 each. As shown by representative IHC photomicrographs (100× magnification), expression of (A) CD11b was found to be markedly elevated in overweight and obese adipose tissue samples as compared with lean samples.
Paraffin-embedded sections (4 μm thick) of subcutaneous adipose tissue were deparaffinized in xylene and rehydrated through descending grades of ethanol (100%, 95%, and 75%) to water. Antigen retrieval was performed under pressure cooker boiling for 8 min and cooling for 15 min. After washing in PBS, endogenous peroxidase activity was blocked with 3% H₂O₂ for 30 min and non-specific antibody binding was clocked with 5% nonfat milk for 1hr and 1% bovine serum albumin (BSA) solution for 1hr. Slides were treated overnight with primary antibodies at room temperature. After washing with PBS (0.5% Tween), slides were incubated for 1hr with secondary antibody conjugated with HRP polymer chain and color was developed using 3,3ʹ-diaminobenzidine chromogen substrate. Specimens were washed in running tap water, lightly counterstained with hematoxylin, dehydrated through ascending grades of ethanol (75%, 95%, and 100%), cleared in xylene, and finally mounted in dibutyl phthalate xylene (DPX).
Image from Martin et al PLoS One. 2014 Feb 5;9(2):e88226. doi: 10.1371/journal.pone.0088226. eCollection 2014. Fig 6.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Duchenne muscular dystrophy (DMD) muscle was co-stained for Neu5Gc (green), ab52478 (red) and DAPI (blue).
For double immunostaining, sections were first stained overnight at 4°C with anti-Neu5Gc after blocking in 10% (Neu5Gc-free) human serum, after blocking in 5 mg/mL BSA, sections were incubated overnight with both primary antibodies without fixation, washed for one hour and incubated with the appropriate secondary antibodies.
Immunoprecipitation - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
ab52478 (purified) at 1:30 dilution (2 μg) immunoprecipitating CD11b in TF-1 (Human bone marrow erythroleukemia cell line) whole cell lysate.
Lane 1: TF-1 whole cell lysate 10 μg (input).
Lane 2: ab52478 + TF-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52478 in TF-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Predicted band size: 127 kDa
Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 0.3 µg/mL
All lanes: TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 127 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue sections labeling CD11b with purified ab52478 at 1:1000 dilution (0.28 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Hematoxylin was used as a counterstain.
Negative control: PBS instead of the primary antibody (inset).
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
Unpurified ab52478 staining CD11b in the THP-1 (Human monocytic leukemia cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI.
Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
All lanes: Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 1/20000 dilution
All lanes: TF-1 (Human bone marrow erythroleukemia cell line) lysate at 10 µg
Secondary
All lanes: goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 127 kDa
Observed band size: 170 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
IHC image of CD11b staining in a formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with unpurified ab52478, 5 μg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre