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AB184307

Anti-CD11b antibody [EPR19373]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CD11b antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

2 Images
Western blot - Anti-CD11b antibody [EPR19373] (AB184307)
  • WB

Unknown

Western blot - Anti-CD11b antibody [EPR19373] (AB184307)

Blocking/Dilution buffer : 5% NFDM/TBST.

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID : 10603370). It is exclusively restricted to cells of the myeloid lineages and monocytes, but not in T or B cell lines, so WEHI-231 cell lysate serves as a negative control (PMID : 2457584).

Lanes 1 - 3:

Western blot - Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution

Lanes 4 - 5:

Western blot - Anti-CD11b antibody [EPR19373] (ab184307) at 1/5000 dilution

Lane 1:

Mouse spleen lysate at 20 µg

Lane 2:

M1 (Mouse myeloblast myeloid leukaemia cell line) whole cell lysate at 20 µg

Lane 3:

WEHI-231 (Mouse lymphoblast B cell lymphoma cell line) whole cell lysate at 20 µg

Lane 4:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 5:

J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 127 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

Western blot - Anti-CD11b antibody [EPR19373] (AB184307)
  • WB

Supplier Data

Western blot - Anti-CD11b antibody [EPR19373] (AB184307)

Blocking/Dilution buffer : 5% NFDM/TBST.

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit (PMID : 10603370).

All lanes:

Western blot - Anti-CD11b antibody [EPR19373] (ab184307) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 127 kDa

Observed band size: 170 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-CD11b antibody [EPR19373] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19373

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Amino acids 57:42 PubMed40844715

2025

IGF2BP1-mediated methylation of ABCA1 facilitates tumor progression by affecting cholesterol metabolism in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Xu,Kai Liu,Zhao Chen,Weijian Tang,Zhoumiao Chen

European journal of pain (London, England) 28:1674-1684 PubMed38982797

2024

Potential analgesic effect of a novel cannabidiol nanocrystals powder for the treatment of neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Xinzhen Fu,Meihua Zheng,Qian Liu,Hailin Gan,Zeyu Song,Mingyan Yang,Ke Liu,Zeping Xie,Huaying Fan

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2309330 PubMed38526158

2024

A Graphene Composite Film Based Wearable Far-Infrared Therapy Apparatus (GRAFT) for Effective Prevention of Postoperative Peritoneal Adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohuan Lu,Luming Xu,Yu Song,Xiangnan Yu,Qilin Li,Feng Liu,Xiaoqiong Li,Jiangbo Xi,Shuai Wang,Lin Wang,Zheng Wang

Science advances 9:eadk1050 PubMed38019917

2023

Biochemically functionalized probes for cell-type-specific targeting and recording in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Anqi Zhang,Theodore J Zwang,Charles M Lieber

Journal of neuroinflammation 19:283 PubMed36434679

2022

Hippocampal microRNA-26a-3p deficit contributes to neuroinflammation and behavioral disorders via p38 MAPK signaling pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Changmin Wang,Ye Li,Yuhang Yi,Guiyu Liu,Ruojing Guo,Liyan Wang,Tian Lan,Wenjing Wang,Xiao Chen,Shihong Chen,Shu Yan Yu
View all publications
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Product promise

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