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AB34216

Anti-CD11b antibody [ICRF44]

5

(2 Reviews)

|

(11 Publications)

Mouse Monoclonal CD11b antibody. Suitable for Flow Cyt, IP, WB, IF and reacts with Pig, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Human samples. Cited in 11 publications. Immunogen corresponding to Cell preparation containing ITGAM protein.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [ICRF44]

  • 519 FITC

    FITC Anti-CD11b antibody [ICRF44]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [ICRF44]

  • 660 APC

    APC Anti-CD11b antibody [ICRF44]

  • 519 FITC

    FITC Anti-CD11b antibody [ICRF44]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [ICRF44]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [ICRF44]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ICRF44

Isotype

IgG1

Carrier free

No

Reacts with

Human, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey

Applications

IF, WB, IP, Flow Cyt

applications

Immunogen

Cell preparation containing ITGAM protein. The exact immunogen used to generate this antibody is proprietary information.

P11215

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Reactivity data

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Properties and storage information

Form
Liquid
Purity
undefined SDS-PAGE
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride, 0.142% Sodium dihydrogen phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37036007

2023

Cannabinoid enhancement of lncRNA MMP25-AS1/MMP25 interaction reduces neutrophil infiltration and intestinal epithelial injury in HIV/SIV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Lakmini S Premadasa,Eunhee Lee,Marina McDew-White,Xavier Alvarez,Sahana Jayakumar,Binhua Ling,Chioma M Okeoma,Siddappa N Byrareddy,Smita Kulkarni,Mahesh Mohan

Arthritis & rheumatology (Hoboken, N.J.) 75:950-960 PubMed36530127

2023

Knee Alignment Correction by High Tibial Osteotomy Reduces Symptoms and Synovial Inflammation in Knee Osteoarthritis Accompanied by Macrophage Phenotypic Change From M1 to M2.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeo Yoshida,Kohei Nishitani,Hiroyuki Yoshitomi,Shinichi Kuriyama,Shinichiro Nakamura,Takayuki Fujii,Motoo Saito,Yu Kobori,Akinori Murakami,Koichi Murata,Hiromu Ito,Hideki Ueno,Shuichi Matsuda

Acta pharmacologica Sinica 44:1277-1289 PubMed36517670

2022

IDO-1 inhibitor INCB24360 elicits distant metastasis of basal extruded cancer cells in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hada Buhe,Ji-Xin Ma,Fang-Zhou Ye,Chen-Yun Song,Xin-Yu Chen,Yang Liu,Huang Lin,Xu Han,Li-Xiang Ma,Hexige Saiyin

Journal of veterinary research 65:307-313 PubMed34917843

2021

A Pilot Study To Establish an Ovalbumin-induced Atopic Dermatitis Minipig Model.

Applications

Unspecified application

Species

Unspecified reactive species

Young Kyu Kim,Ju Young Lee,Jeong Ho Hwang,Han Na Suh

Experimental and therapeutic medicine 20:736-747 PubMed32742319

2020

STIM1 controls calcineurin/Akt/mTOR/NFATC2-mediated osteoclastogenesis induced by RANKL/M-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjiao Huang,Qiang Li,Zunyong Feng,Lanrong Zheng

The international journal of biochemistry & cell b 114:105560 PubMed31220583

2019

Kaposi Sarcoma Herpes Virus (KSHV) infection inhibits macrophage formation and survival by counteracting Macrophage Colony-Stimulating Factor (M-CSF)-induced increase of Reactive Oxygen Species (ROS), c-Jun N-terminal kinase (JNK) phosphorylation and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Saveria Gilardini Montani,Luca Falcinelli,Roberta Santarelli,Maria Anele Romeo,Marisa Granato,Alberto Faggioni,Mara Cirone

Journal of neuropathology and experimental neurology 78:492-500 PubMed31058279

2019

Tissue Transglutaminase Appears in Monocytes and Macrophages but Not in Lymphocytes in White Matter Multiple Sclerosis Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Navina L Chrobok,John G J M Bol,Micha M M Wilhelmus,Benjamin Drukarch,Anne-Marie van Dam

Journal of Alzheimer's disease : JAD 68:695-710 PubMed30883351

2019

Modulation of SPARC/Hevin Proteins in Alzheimer's Disease Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Strunz,Juliet T Jarrell,David S Cohen,Eric R Rosin,Charles R Vanderburg,Xudong Huang

Nature biomedical engineering 2:810-821 PubMed30873298

2018

Alginate encapsulation as long-term immune protection of allogeneic pancreatic islet cells transplanted into the omental bursa of macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew A Bochenek,Omid Veiseh,Arturo J Vegas,James J McGarrigle,Meirigeng Qi,Enza Marchese,Mustafa Omami,Joshua C Doloff,Joshua Mendoza-Elias,Mohammad Nourmohammadzadeh,Arshad Khan,Chun-Chieh Yeh,Yuan Xing,Douglas Isa,Sofia Ghani,Jie Li,Casey Landry,Andrew R Bader,Karsten Olejnik,Michael Chen,Jennifer Hollister-Lock,Yong Wang,Dale L Greiner,Gordon C Weir,Berit Løkensgard Strand,Anne Mari A Rokstad,Igor Lacik,Robert Langer,Daniel G Anderson,Jose Oberholzer

Oncology letters 16:5398-5404 PubMed30250610

2018

High expression of S100A12 on intratumoral stroma cells indicates poor prognosis following surgical resection of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Cai,Bo-Gen Ye,Jian-Yang Ao,Xiao-Dong Zhu,Yuan-Yuan Zhang,Zong-Tao Chai,Cheng-Hao Wang,Hui-Chuan Sun
View all publications
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