Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free
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(1 Publication)
Mouse Monoclonal CD11b antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITGAM aa 900-1100.
View Alternative Names
CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free (AB269686)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab269676 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269676).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free (AB269686)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab269676 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269676).
- WB
Unknown
Western blot - Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free (AB269686)
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269676).
All lanes:
Western blot - Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free (ab269686) at 2 µg/mL
All lanes:
Human spleen lysate
Predicted band size: 127 kDa
false
- Protein Array
Unknown
Protein Array - Anti-CD11b antibody [ITGAM/3338] - BSA and Azide free (AB269686)
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269676.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269676).
Reactivity data
Product details
ab269686 is the carrier-free version of ab269676.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.
Pathways
CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.
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Target data
Publications (1)
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Cancer cell international 24:121 PubMed38555451
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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