Anti-CD11b antibody [M1/70] (ab8878) is a rat monoclonal antibody detecting CD11b in Flow Cytometry, ICC/IF. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications
- Trusted since 2002
Images
![Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408115.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--immunocytochemistry-immunofluorescence-img408113.png/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--immunocytochemistry-immunofluorescence-img428725.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)")
![Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408271.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)")
![Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408114.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)")
Publications
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- Molecular neurodegeneration 18:952023Translational profiling identifies sex-specific metabolic and epigenetic reprogramming of cortical microglia/macrophages in APPPS1-21 mice with an antibiotic-perturbed-microbiome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShabana M Shaik,Yajun Cao,Joseph V Gogola,Hemraj B Dodiya,Xulun Zhang,Hejer Boutej,Weinong Han,Jasna Kriz,Sangram S SisodiaPubMed 38104136
- BMC medical genomics 16:3142023Key mA regulators mediated methylation modification pattern and immune infiltration characterization in hepatic ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixi Zhang,Can Qi,Yiwen Guo,Xuefeng Li,Zebin ZhuPubMed 38049811
- Advanced materials (Deerfield Beach, Fla.) 35:e23028012023Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanyi Huang,Yawei Du,Yanjia Chen,Zeping Qiu,Zhiyan Wang,Yingze Fan,Yunjing Shi,Zhuojin Li,Ke Yang,Wenguo Cui,Wei JinPubMed 37589156
- Clinical and translational medicine 13:e13832023Nme Cas9-mediated therapeutic editing in inhibiting angiogenesis after wet age-related macular degeneration onset.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSihui Hu,Yuxi Chen,Dongchun Xie,Kan Xu,Yunzhao Fu,Wei Chi,Haiying Liu,Junjiu HuangPubMed 37598400
- Scientific reports 13:88562023Next-generation RNA sequencing elucidates transcriptomic signatures of pathophysiologic nerve regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWesley S Warner,Christopher Stubben,Stewart Yeoh,Alan R Light,Mark A MahanPubMed 37258605
- JCI insight 8:2023Thyroid hormone modulates hyperoxic neonatal lung injury and mitochondrial function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBianca M Vamesu,Teodora Nicola,Rui Li,Snehashis Hazra,Sadis Matalon,Naftali Kaminski,Namasivayam Ambalavanan,Jegen KandasamyPubMed 36917181
- Glia 71:1804-18292023Microglia actively remove NR1 autoantibody-bound NMDA receptors and associated post-synaptic proteins in neuron microglia co-cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazi Atikur Rahman,Marta Orlando,Ayub Boulos,Ewa Andrzejak,Dietmar Schmitz,Noam E Ziv,Harald Prüss,Craig C Garner,Aleksandra IchkovaPubMed 37026600
- Frontiers in immunology 14:11396012023γδ T cells recruitment and local proliferation in brain parenchyma benefit anti-neuroinflammation after cerebral microbleeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Su,Shuxian Yang,Yanxiang Li,Zongqin Xiang,Qiao Tao,Shengde Liu,Zhinan Yin,Liyun Zhong,Xiaoxu Lv,Libing ZhouPubMed 37063908
- Cells 12:2023Cirrhotic Liver Sustains In Situ Regeneration of Acellular Liver Scaffolds after Transplantation into G-CSF-Treated Animals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarlon Lemos Dias,Inês Julia Ribas Wajsenzon,Gabriel Bastos Naves Alves,Bruno Andrade Paranhos,Cherley Borba Vieira Andrade,Victoria Regina Siqueira Monteiro,Raysa Maria Reis de Sousa,Evelyn Nunes Goulart da Silva Pereira,Karine Lino Rodrigues,Anissa Daliry,Debora Bastos Mello,Regina Coeli Dos Santos GoldenbergPubMed 37048049
- Cell & bioscience 13:82023Photobiomodulation improves the synapses and cognitive function and ameliorates epileptic seizure by inhibiting downregulation of Nlgn3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamgue Hong,Hee Jung Kim,Keunsoo Kang,Ji On Park,Seyoung Mun,Hyung-Gun Kim,Bong Hui Kang,Phil-Sang Chung,Min Young Lee,Jin-Chul AhnPubMed 36635704
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Alternative names
CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor
Recommended products
Anti-CD11b antibody [M1/70] (ab8878) is a rat monoclonal antibody detecting CD11b in Flow Cytometry, ICC/IF. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 150 publications
- Trusted since 2002
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- M1/70
- Purification technique
- Affinity purification Protein A
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD11b antibody [M1/70] (ab8878) is a rat recombinant monoclonal antibody and is validated for use in Flow Cyt, ICC/IF in mouse, rat samples.
Anti-CD11b antibody [M1/70] (ab8878) has been cited over 158 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-CD11b antibody [M1/70] (ab8878) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD11b antibody [M1/70] (ab8878) has 14 independent reviews from customers.
Anti-CD11b antibody [M1/70] (ab8878) specifically detects CD11b (UniProt ID: P05555; Molecular weight: 126kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).
Conjugation-ready, carrier free format available for antibody clone M1/70 - Anti-CD11b antibody [M1/70] - BSA and Azide free ab237965.
Antibody clone M1/70 is also available pre-conjugated to a variety of labels for your convenience - FITC, PE, APC, PE/Cy5®, APC/Cy7®, Alexa Fluor® 488, Alexa Fluor® 647, PerCP/Cy5.5® (FITC Anti-CD11b antibody [M1/70] ab24874, PE Anti-CD11b antibody [M1/70] ab25175, APC Anti-CD11b antibody [M1/70] ab25482, PE/Cy5® Anti-CD11b antibody [M1/70] ab25533, APC/Cy7® Anti-CD11b antibody [M1/70] ab79096, Alexa Fluor® 488 Anti-CD11b antibody [M1/70] ab197701, Alexa Fluor® 647 Anti-CD11b antibody [M1/70] ab197702, PerCP/Cy5.5® Anti-CD11b antibody [M1/70] ab210299).
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
- Biological function summary
CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.
- Pathways
CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.
- Associated diseases and disorders
CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408115.png "Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)")
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--immunocytochemistry-immunofluorescence-img408113.png "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)")
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
CD11b Immunocytochemistry/ Immunofluorescence staining using rat Anti-CD11b antibody
Confocal image showing membranous staining in RAW 264.7 cells.
RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Negative control: WEHI-231 (PMID: 2457584)![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--immunocytochemistry-immunofluorescence-img428725.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)")
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte cells labelling LY6C with Anti-LY6C antibody [EPR27220-67] ab305229 at a 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at a 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. Nuclear DNA was labeled with DAPI (shown in blue).
Counterstained with Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/1000 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160) at a 1/1000 dilution (shown in magenta).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The negative controls are as follows:
-ve control 1: LY6C (Anti-LY6C antibody [EPR27220-67] ab305229) at a 1/100 dilution, followed by Goat anti-Rat IgG H&L (Alexa Fluor® 594) (Goat Anti-Rat IgG H&L (Alexa Fluor® 594) ab150160) secondary at a 1/1000 dilution.
-ve control 2: Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at a 1/1000 dilution.![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408271.png "Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)")
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: WEHI-231.(PMID: 2457584)
Gated on viable cells.![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-ab8878--flow-cytometry-img408114.png "Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)")
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)
Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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