Anti-CD11b antibody [M1/70]

• Flow Cyt

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Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

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Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse bone marrow cells labelling Lactoferrin with ab325119 at 1/50000 (0.01 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subset of CD11b+mouse bone marrows (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain CD11b at 1/200 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. (Magenta).

-ve control 1 : ab325119 at 1/50000 dilution, followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• Flow Cyt

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Flow Cytometry - Anti-CD11b antibody [M1/70] (AB8878)

Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Negative control : WEHI-231.(PMID : 2457584) Gated on viable cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling PGRPS with ab325131 at 1/1000 (0.522 μg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in mouse CD11B+ bone marrow cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Mouse splenocyte cells labelling LY6C with ab305229 at a 1/100 dilution, followed by Goat Anti-Rabbit (Alexa Fluor^® 488) (ab150081) secondary antibody at a 1/1000 dilution. Confocal image showing cytoplasmic staining in subsets of mouse splenocytes (shown in green), which is partially co-located with CD11b. Nuclear DNA was labeled with DAPI (shown in blue).

Counterstained with Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/1000 dilution, followed by Goat Anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) at a 1/1000 dilution (shown in magenta).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The negative controls are as follows :

-ve control 1 : LY6C (ab305229) at a 1/100 dilution, followed by Goat anti-Rat IgG H&L (Alexa Fluor^® 594) (ab150160) secondary at a 1/1000 dilution.

-ve control 2 : Anti-CD11b Rat monoclonal antibody (ab8878) at a 1/200 dilution, followed by Goat anti-Rabbit (Alexa Fluor^® 488) (ab150081) at a 1/1000 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Confocal image showing membranous staining in RAW 264.7 cells. RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1 : 50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI. Negative control : WEHI-231 (PMID : 2457584)

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (AB8878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling Proteinase 3/Myeloblastin with ab324528 at 1/200 (2.445 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in CD11b+ cells of mouse bone marrow (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab324528 at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab8878 at 1/200 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.