Rat Recombinant Monoclonal CD11b antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--immunocytochemistry-immunofluorescence-img408113.png/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965)")
![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408271.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965)")
![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408115.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965)")
![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408114.png/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (AB237965)")
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:20008295, PubMed:9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
Alternative names
CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor
Recommended products
Rat Recombinant Monoclonal CD11b antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- M1/70
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab237965 is the carrier-free version of Anti-CD11b antibody [M1/70] ab8878.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
- Biological function summary
CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.
- Pathways
CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.
- Associated diseases and disorders
CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--immunocytochemistry-immunofluorescence-img408113.png "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)")
Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
CD11b Immunocytochemistry/ Immunofluorescence staining using rat Anti-CD11b antibody
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD11b antibody [M1/70] ab8878)
Confocal image showing membranous staining in RAW 264.7 cells.
RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-CD11b antibody [M1/70] ab8878 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] - Loading Control ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-CD11b antibody [M1/70] ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Negative control: WEHI-231 (PMID: 2457584)![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408271.png "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)")
Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD11b antibody [M1/70] ab8878).
Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using Anti-CD11b antibody [M1/70] ab8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: WEHI-231.(PMID: 2457584)
Gated on viable cells.![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408115.png "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)")
Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD11b antibody [M1/70] ab8878).
Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with Anti-CD11b antibody [M1/70] ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.![Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965), expandable thumbnail](https://content.abcam.com/products/images/cd11b-antibody-m1-70-bsa-and-azide-free-ab237965--flow-cytometry-img408114.png "Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)")
Flow Cytometry - Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CD11b antibody [M1/70] ab8878).
Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with Anti-CD11b antibody [M1/70] ab8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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