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AB224805

Anti-CD11b antibody [SP330] - C-terminal

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal CD11b antibody. C-terminal. Suitable for IHC-P, Flow Cyt, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CD11b, CD11B, CR3A, ITGAM, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human spleen tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Flow cytometric analysis of TF-1 (human bone marrow erythroleukemia cell line) cell line labeling CD11b with ab224805 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue) .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human lung tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

Formalin-fixed, paraffin-embedded human colon tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CD11b antibody [SP330] - C-terminal (AB224805)
  • WB

Supplier Data

Western blot - Anti-CD11b antibody [SP330] - C-terminal (AB224805)

All lanes:

Western blot - Anti-CD11b antibody [SP330] - C-terminal (ab224805) at 1/100 dilution

All lanes:

TF-1 (human bone marrow erythroleukemia cell line) cell lysate

Predicted band size: 127 kDa

false

  • Carrier free

    Anti-CD11b antibody [SP330] - BSA and Azide free

  • 660 APC

    APC Anti-CD11b antibody [SP330] (C-terminal)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [SP330] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD11b antibody [SP330] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD11b antibody [SP330] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [SP330] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [SP330] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD11b antibody [SP330] - C-terminal

  • HRP

    HRP Anti-CD11b antibody [SP330] (C-terminal)

  • 578 PE

    PE Anti-CD11b antibody [SP330] (C-terminal)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP330

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary incubation for 10 minutes at room temperature.</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p>Primary incubation for 30 minutes at at 4°C.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400", "WB-species-notes": "<p>Primary incubation for 1 hour at room temperature.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed : 20008295, PubMed : 9558116). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen and factor X. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed : 28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed : 21193407). Integrin ITGAM/ITGB2 is also a receptor for ICAM1 ligand ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).
See full target information ITGAM
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 30:456 PubMed40762017

2025

Acute myeloid leukemia with plasmacytoid dendritic cell proliferation: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Pei,Yi Zhang,Han Xu,Pei Pei,Zhengyang Zhang,Hongxia Wang,Bei Zhang,Junjun Bai,Yingxin Zhao,Jingjing Gu,Zhiyu Fang,Miaomiao Liu,Qinglin Song

Discover oncology 16:1419 PubMed40720004

2025

BRD8 inhibits colorectal cancer progression through TGF-β1-mediated tumor-associated macrophage recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Xu,Yejia Mo,Bingqi Yu,Sunfu Fan

BMC cardiovascular disorders 25:141 PubMed40021969

2025

Identification of the CeRNA axis of circ_0000006/miR-483-5p/KDM2B in the progression of aortic aneurysm to aorta dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Liu,Xiong Tan,Liang Wang,Weitao Jin,Yangchen He,Yu Yan,Kai Hu,Hao Wang,Chaohu Xiang,Ming Hou,Yinglong Lai

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

Cancers 16: PubMed38398138

2024

Characterizing the Inflammatory Profile of Neutrophil-Rich Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fatma Al Qutami,Walaa AlHalabi,Aswathy Vijayakumar,Surendra Singh Rawat,Abubakr H Mossa,Manju Nidagodu Jayakumar,Baila Samreen,Mahmood Y Hachim

BMC pharmacology & toxicology 23:81 PubMed36273189

2022

Dexmedetomidine alleviates inflammatory response and oxidative stress injury of vascular smooth muscle cell via α2AR/GSK-3β/MKP-1/NRF2 axis in intracranial aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Zhang,Xiue Mu,Xiaohui Zhou

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 32:1495-1507 PubMed31186528

2019

Characterization of spatial distribution of tumor-infiltrating CD8 T cells refines their prognostic utility for pancreatic cancer survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Masugi,Tokiya Abe,Akihisa Ueno,Yoko Fujii-Nishimura,Hidenori Ojima,Yutaka Endo,Yusuke Fujita,Minoru Kitago,Masahiro Shinoda,Yuko Kitagawa,Michiie Sakamoto
View all publications
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Product promise

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