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AB238658

Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal CD11b + CD11c antibody. Carrier free. Suitable for IHC-Fr, ICC/IF and reacts with Rat samples. Cited in 1 publication.

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Itgam

1 Images
Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (AB238658)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (AB238658)

This data was developed using ab1211, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of CD11b + CD11c staining in a section of 10% formalin fixed (10 mins, RT) frozen rat spleen.

Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab1211 at 1μg/ml. The section was then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1.5μg/ml) for 1 hour at room temperature. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX42

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab238658 is the carrier-free version of ab1211.

IHC protocol advice:

This antibody is not suitable for IHC on paraffin-embedded samples.

For best results in IHC on frozen tissue, the following may help detection:
1) Paraformaldehyde** perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) Use OCT (TissueTek) compound for embedding.
5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
6) Do not heat the samples or sections.**
7) During the staining procedure, do not allow the sections to dry out.

Our Technical team (technical@abcam.com) will be happy to provide further information and advice.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b and CD11c also known as integrins αM and αX respectively are important proteins in the immune system. CD11b with a molecular mass of approximately 165 kDa and CD11c around 150 kDa form integrin complexes with CD18. These proteins are expressed on various immune cells including macrophages monocytes granulocytes and dendritic cells. Their expression can also be detected in a subset of B and T cells. The antibody against CD11b also termed OX-42 is often used in research to identify immune cells that express this surface protein.
Biological function summary

Integrins αM (CD11b) and αX (CD11c) facilitate key processes such as adhesion and migration of leukocytes. Both CD11b and CD11c pair with CD18 to form the Mac-1 (Macrophage-1 antigen also known as CR3) and p15095 complexes respectively. These complexes participate in both innate and adaptive immune responses by binding to various ligands like ICAM-1 and fibrinogen. CD11b/CD18 (Mac-1) additionally plays an essential role in phagocytosis degranulation and respiratory burst important activities for pathogen clearance.

Pathways

These integrins engage in critical signaling pathways including the NF-kappaB and MAPK pathways. They regulate the inflammatory response and cell survival. CD11b interacts closely with other proteins like ICAM-1 and C3b which are involved in these pathways. CD11c also contributes to similar pathways although it plays a more specialized role in dendritic cells impacting antigen presentation and immune regulation.

CD11b and CD11c have associations with conditions like autoimmune diseases and chronic inflammatory disorders. Both proteins show altered expression levels in diseases such as rheumatoid arthritis and systemic lupus erythematosus. For instance in these diseases the Mac-1 complex (CD11b/CD18) frequently exhibits dysregulation affecting leukocyte adhesion and migration. The expression of CD11c is modified in certain leukemias and lymphomas where it correlates with disease progression and prognosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information CD11b

Additional targets

CD11c
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 19:47 PubMed38862989

2024

Single molecule array measures of LRRK2 kinase activity in serum link Parkinson's disease severity to peripheral inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yuan,Huizhong Li,Kashyap Sreeram,Tuyana Malankhanova,Ravindra Boddu,Samuel Strader,Allison Chang,Nicole Bryant,Talene A Yacoubian,David G Standaert,Madalynn Erb,Darren J Moore,Laurie H Sanders,Michael W Lutz,Dmitry Velmeshev,Andrew B West
View all publications
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Product promise

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