Anti-CD11c antibody [EPR1346] - BSA and Azide free

• WB

Unknown

Western blot - Anti-CD11c antibody [EPR1346] - BSA and Azide free (AB248421)

This data was developed using ab133246, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CD11c antibody [EPR1346] (<a href='/en-us/products/primary-antibodies/cd11c-antibody-epr1346-ab133246'>ab133246</a>) at 1/1000 dilution

Lane 1:

HL60 cell lysate, TPA stimulated at 10 µg

Lane 2:

THP1 cell lysate, TPA stimulated at 10 µg

Predicted band size: 127 kDa

false

• WB

Lab

Western blot - Anti-CD11c antibody [EPR1346] - BSA and Azide free (AB248421)

This data was developed using ab133246, the same antibody clone in a different buffer formulation.

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-CD11c antibody [EPR1346] (<a href='/en-us/products/primary-antibodies/cd11c-antibody-epr1346-ab133246'>ab133246</a>) at 1/1000 dilution

Lane 1:

THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg

Lane 2:

THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 127 kDa

Observed band size: 150 kDa

true

Exposure time: 12min