AB212508

Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free

Name: Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free (ab212508) | Abcam
Brand: Abcam Limited
SKU: AB212508
Price: 905 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(2 Publications)

Mouse Monoclonal CD11c antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITGAX aa 600-850.

View Alternative Names

CD11c, CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free (AB212508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free (AB212508)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Tonsil tissue labeling CD11c with ab212508 at 1ug/ml.

Protein Array - Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free (AB212508)

Protein Array

Unknown

Protein Array - Anti-CD11c antibody [ITGAX/1242] - BSA and Azide free (AB212508)

This data was produced with ab216028, the same antibody in a different formulation with BSA and Azide.
ab216028 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ITGAX/1242

Isotype

IgG

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human ITGAX aa 600-850. The exact immunogen used to generate this antibody is proprietary information.

P20702

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.

Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

See full target information ITGAX

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 35:1209-1226.e13 PubMed37172577

2023

Extracellular vesicles in fatty liver promote a metastatic tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhijun Wang,So Yeon Kim,Wei Tu,Jieun Kim,Alexander Xu,Yoon Mee Yang,Michitaka Matsuda,Lien Reolizo,Takashi Tsuchiya,Sandrine Billet,Alexandra Gangi,Mazen Noureddin,Ben A Falk,Sungjin Kim,Wei Fan,Mourad Tighiouart,Sungyong You,Michael S Lewis,Stephen J Pandol,Dolores Di Vizio,Akil Merchant,Edwin M Posadas,Neil A Bhowmick,Shelly C Lu,Ekihiro Seki

PloS one 12:e0173017 PubMed28278185

2017

Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Yamauchi,Masafumi Moriyama,Jun-Nosuke Hayashida,Takashi Maehara,Noriko Ishiguro,Keigo Kubota,Sachiko Furukawa,Miho Ohta,Mizuki Sakamoto,Akihiko Tanaka,Seiji Nakamura

View all publications

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