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AB254183

Anti-CD11c antibody [KB90]

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(17 Publications)

Anti-CD11c antibody [KB90] (ab254183) is a mouse monoclonal antibody detecting CD11c in Flow Cytometry. Suitable for Human, Mouse.

View Alternative Names

CD11c, CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5

4 Images
Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183)
  • Flow Cyt

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Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183)

Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.

Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

The negative controls are as follows :
-ve control 1 : ab254183 at 1/50 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183)

Flow cytometric analysis of mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

KB90

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/800", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/800", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-CD11c antibody [KB90] (ab254183) is a mouse recombinant monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human, Mouse samples.

Other related products
We have a range of other formats of antibody clone [KB90] also available for your convenience: ab254183, Carrier free - ab255937, Alexa Fluor® 488 - ab313900, Alexa Fluor® 594 - ab313901, Alexa Fluor® 647 - ab313902, PE - ab316176

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
See full target information ITGAX
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed41070609

2025

Ginsenoside Rh1 inhibits tumor growth in mice with colorectal cancer and depressive symptoms via modulation of the gut microbiota and tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Dai,Lingchang Li,Jiaxin Li,Xiaobin Tan,Yi Luo,Xuedong Wang,Jie Song,Sujuan Li,Songshan Shi

Frontiers in immunology 16:1649891 PubMed40977690

2025

FLT3L combined with GM-CSF induced dendritic cells drive broad tumor-specific CD8 T cell responses and remodel the tumor microenvironment to enhance anti-tumor efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zheng,Jiajie Zhang,He Sui,Yu Sun,Ningning Lv,Lin Liu,Ming Qu,Jiateng Tan,Bin Zhang,Zhanhao Mo

Materials today. Bio 33:102045 PubMed40688666

2025

Dendritic cell membrane-based DCsLipo@MnO@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye

Frontiers in immunology 16:1576069 PubMed40589746

2025

Cordycepin, lactoferrin, and Sargassum fusiforme polysaccharides protects against RSV via M2-like macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozong Fu,Panwen Huang,Yuting Zhang,Yanchang Li,Shichang Hu

Oncology letters 28:468 PubMed39119236

2024

Myeloid/lymphoid neoplasms with eosinophilia and FGFR1 rearrangement t(8;13)(p11;q12): A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Guo,Meng-Xue Ma,Tian Tian,Jing-Nan Zhang,Xiao-Nan Guo,Shukai Qiao

British journal of pharmacology 181:3760-3778 PubMed38872396

2024

Factor XII and prekallikrein promote microvascular inflammation and psoriasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Zhang,Zengrong Chen,Junyan Guo,Qing Wan,Yingjie Zhang,Huihui Li,Haojie Rao,Jianfeng Yang,Pengfei Xu,Hong Chen,Miao Wang

Journal of inflammation research 17:1687-1706 PubMed38504693

2024

Envoplakin Inhibits Macrophage Polarization by Altering the Inflammatory Tumor Microenvironment of Melanoma Through the RAS / ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weilin Cai,Minliang Chen

Nature communications 15:1688 PubMed38402201

2024

Fusobacterium nucleatum promotes tumor progression in KRAS p.G12D-mutant colorectal cancer by binding to DHX15.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Zhu,Man Li,Dexi Bi,Huiqiong Yang,Yaohui Gao,Feifei Song,Jiayi Zheng,Ruting Xie,Youhua Zhang,Hu Liu,Xuebing Yan,Cheng Kong,Yefei Zhu,Qian Xu,Qing Wei,Huanlong Qin

Biomaterials research 26:77 PubMed36494759

2022

Ginsenoside Rg3 nanoparticles with permeation enhancing based chitosan derivatives were encapsulated with doxorubicin by thermosensitive hydrogel and anti-cancer evaluation of peritumoral hydrogel injection combined with PD-L1 antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Guoli Wei,Lixia Luo,Lingchang Li,Yibo Gao,Xiaobin Tan,Sen Wang,Haoxiao Chang,Yuxi Liu,Yingjie Wei,Jie Song,Zhenhai Zhang,Jiege Huo

Journal of extracellular vesicles 11:e12289 PubMed36468941

2022

Biosynthetic neoantigen displayed on bacteria derived vesicles elicit systemic antitumour immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqiang Meng,Liyan Li,Zhirang Zhang,Zhongda Lin,Jinxie Zhang,Xiao Song,Tianyuan Xue,Chenyang Xing,Xin Liang,Xudong Zhang
View all publications
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