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Mouse Recombinant Monoclonal CD11c antibody. Suitable for ICC, Flow Cyt and reacts with Mouse, Human samples. Cited in 6 publications.

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Images

Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183), expandable thumbnail
  • Immunocytochemistry - Anti-CD11c antibody [KB90] (AB254183), expandable thumbnail
  • Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183), expandable thumbnail
  • Flow Cytometry - Anti-CD11c antibody [KB90] (AB254183), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWBIHC-P
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/800
Notes

-

Species
Human
Dilution info
1/800
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

Alternative names

CD11c, CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5

Recommended products

Mouse Recombinant Monoclonal CD11c antibody. Suitable for ICC, Flow Cyt and reacts with Mouse, Human samples. Cited in 6 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
KB90
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.

Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

Associated diseases and disorders

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183), expandable thumbnail

    Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

  • Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183), expandable thumbnail

    Immunocytochemistry - Anti-CD11c antibody [KB90] (ab254183)

    Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

    The negative controls are as follows:
    -ve control 1: ab254183 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
    -ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

  • Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183), expandable thumbnail

    Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

    Flow cytometric analysis of mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

  • Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183), expandable thumbnail

    Flow Cytometry - Anti-CD11c antibody [KB90] (ab254183)

    Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113), at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

    Gated on viable cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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