Anti-CD11c antibody [KB90] - BSA and Azide free

• Flow Cyt

Ap

Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (AB255937)

Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

• ICC

Supplier Data

Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (AB255937)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

• ICC

Supplier Data

Immunocytochemistry - Anti-CD11c antibody [KB90] - BSA and Azide free (AB255937)

Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with ab254183 at 1/100 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).

The negative controls are as follows :
-ve control 1 : ab254183 at 1/50 dilution, followed by ab150080 AlexaFluor^®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11c antibody [KB90] - BSA and Azide free (AB255937)

Flow cytometric analysis of mouse splenocytes labeling CD11c with ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with mouse IgG (Left) or ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254183).