Mouse Recombinant Monoclonal CD11c antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Mouse, Human samples.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ICC | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended |
Mouse | Tested | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/800 | Notes - |
Species Human | Dilution info 1/800 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
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Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
CD11C, ITGAX, Integrin alpha-X, CD11 antigen-like family member C, Leu M5
Mouse Recombinant Monoclonal CD11c antibody. Carrier free. Suitable for ICC, Flow Cyt and reacts with Mouse, Human samples.
IgG1
Mouse
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
KB90
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab255937 is the carrier-free version of Anti-CD11c antibody [KB90] ab254183.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.
CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.
CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) (either untreated or treated with 400 ng/mL PMA for 96 hours) cells labeling CD11c with Anti-CD11c antibody [KB90] ab254183 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in THP-1 cells treated with PMA (400ng/mL, 96h). The nuclear counter stain is DAPI (Blue). Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD11c antibody [KB90] ab254183).
Immunocytochemistry analysis of 4% parafomaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes labeling CD11c with Anti-CD11c antibody [KB90] ab254183 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in subsets of mouse splenocytes . The nuclear counter stain is DAPI (Blue). Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Red).
The negative controls are as follows:
-ve control 1: Anti-CD11c antibody [KB90] ab254183 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD11c antibody [KB90] ab254183).
Flow cytometric analysis of mouse splenocytes labeling CD11c with Anti-CD11c antibody [KB90] ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or Anti-CD11c antibody [KB90] ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD11c antibody [KB90] ab254183).
Flow cytometric analysis of human PBMCs (peripheral blood mononuclear cells) labeling CD11c with Anti-CD11c antibody [KB90] ab254183 at 1/800 dilution (Right panel) compared with an Isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113), at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or Anti-CD11c antibody [KB90] ab254183 (Right). Then stained with anti-CD11b conjugated to BV421.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD11c antibody [KB90] ab254183).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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