JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB33483

Anti-CD11c antibody [N418]

4

(14 Reviews)

|

(96 Publications)

Armenian hamster Monoclonal CD11c antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 96 publications.

View Alternative Names

CD11c, Integrin alpha-X, CD11 antigen-like family member C, Itgax

2 Images
Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)
  • Flow Cyt

AbReview50886****

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)

Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)

Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11c antibody [N418]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11c antibody [N418]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11c antibody [N418]

  • 660 APC

    APC Anti-CD11c antibody [N418]

  • 519 FITC

    FITC Anti-CD11c antibody [N418]

  • 578 PE

    PE Anti-CD11c antibody [N418]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11c antibody [N418]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11c antibody [N418]

  • Biotin

    Biotin Anti-CD11c antibody [N418]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11c antibody [N418]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11c antibody [N418]

  • Carrier free

    Anti-CD11c antibody [N418] - BSA and Azide free

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

N418

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB264107

Anti-CD11c antibody [3.9] - BSA and Azide free

Flow Cytometry - Anti-CD11c antibody [3.9] - BSA and Azide free (AB264107)

  • 0
  • 0
Primary Antibodies

AB11029

Anti-CD11c antibody [3.9]

Trial Size

Flow Cytometry - Anti-CD11c antibody [3.9] (AB11029)

  • 4
  • 11
Primary Antibodies

AB195534

HRP Anti-CD11c antibody [EP1347Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD11c antibody [EP1347Y] (AB195534)

  • 0
  • 0
Primary Antibodies

AB240558

Anti-CD11c antibody [EPR21826] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD11c antibody [EPR21826] - BSA and Azide free (AB240558)

  • 4
  • 1
Primary Antibodies

AB216655

Anti-CD11c antibody [EP1347Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - BSA and Azide free (AB216655)

  • 4
  • 11
Primary Antibodies

AB133246

Anti-CD11c antibody [EPR1346]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-CD11c antibody [EPR1346] (AB133246)

  • 0
  • 0
Primary Antibodies

AB219799

Anti-CD11c antibody [EPR21826]

20ul selling size|RabMAb|Recombinant

Flow Cytometry - Anti-CD11c antibody [EPR21826] (AB219799)

  • 4
  • 7
Primary Antibodies

AB52632

Anti-CD11c antibody [EP1347Y] - C-terminal

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

  • 5
  • 18

Complementary Products

Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB217344

Anti-CD8 alpha antibody [EPR21769]

RabMAb|Recombinant

Flow Cytometry - Anti-CD8 alpha antibody [EPR21769] (AB217344)

  • 5
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Functions as a receptor of the erythrocyte-specific adhesion molecule ICAM4 and mediates erythrophagocytosis.
See full target information Itgax
style
left-column

Publications (96)

Recent publications for all applications. Explore the full list and refine your search

Open veterinary journal 15:3558-3570 PubMed41036017

2025

Host-parasite interaction in latent cerebral toxoplasmosis: The role of cytokine response in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Tuğçe Anteplioğlu,Merve Bişkin Türkmen,Erva Eser,Damla Okatan,Oğuz Kul

PloS one 20:e0318107 PubMed39932994

2025

Macrophage-P2X4 receptors pathway is essential to persistent inflammatory muscle hyperalgesia onset, and is prevented by physical exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Graciana de Azambuja,Fernando Moreira Simabuco,Maria Cláudia Gonçalves de Oliveira

Nutrients 16: PubMed39408256

2024

Memory T-Cells Contribute to Calcium Release from Bones during Lactation in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Anna Cline-Smith,Brady Chrisler,Brittani Lubeck,Ajit Perla,Sumona Banerjee,Ida Fan,Rajeev Aurora

Scientific reports 14:21126 PubMed39256539

2024

Maid gene dysfunction promotes hyperobesity via the reduction of adipose tissue inflammation in Mc4r gene-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kyutaro Koyama,Akira Sakamaki,Shinichi Morita,Itsuo Nagayama,Marina Kudo,Yuto Tanaka,Naruhiro Kimura,Yoshihisa Arao,Hiroyuki Abe,Kenya Kamimura,Shuji Terai

Cell reports. Medicine 5:101528 PubMed38677283

2024

Exogenous non-coding dsDNA-dependent trans-activation of phagocytes augments anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Tiphaine Delaunay,Sehee Son,Seongji Park,Balveen Kaur,Jeonghyun Ahn,Glen N Barber

eLife 12: PubMed38127424

2023

Apoptosis recognition receptors regulate skin tissue repair in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie Horsley

Bioactive materials 32:12-27 PubMed37790917

2023

immunomodulation of tumors with biosynthetic bacteria promote anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongda Lin,Fanqiang Meng,Yumeng Ma,Chi Zhang,Zhirang Zhang,Zhaoxin Yang,Yuan Li,Linlin Hou,Yuzhong Xu,Xin Liang,Xudong Zhang

JCI insight 8: PubMed36917181

2023

Thyroid hormone modulates hyperoxic neonatal lung injury and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca M Vamesu,Teodora Nicola,Rui Li,Snehashis Hazra,Sadis Matalon,Naftali Kaminski,Namasivayam Ambalavanan,Jegen Kandasamy

Cell reports methods 3:100398 PubMed36936072

2023

Label-free cell segmentation of diverse lymphoid tissues in 2D and 3D.

Applications

Unspecified application

Species

Unspecified reactive species

John W Wills,Jack Robertson,Pani Tourlomousis,Clare M C Gillis,Claire M Barnes,Michelle Miniter,Rachel E Hewitt,Clare E Bryant,Huw D Summers,Jonathan J Powell,Paul Rees

In vivo (Athens, Greece) 37:132-142 PubMed36593019

2023

Age-related Changes in Trigeminal Ganglion Macrophages Enhance Orofacial Ectopic Pain After Inferior Alveolar Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Fujiwara,Kentaro Urata,Tatsuki Oto,Yoshinori Hayashi,Suzuro Hitomi,Koichi Iwata,Toshimitsu Iinuma,Masamichi Shinoda
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com