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AB33483

Anti-CD11c antibody [N418]

4

(14 Reviews)

|

(81 Publications)

Armenian hamster Monoclonal CD11c antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 81 publications.

View Alternative Names

CD11c, Integrin alpha-X, CD11 antigen-like family member C, Itgax

2 Images
Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)
  • Flow Cyt

AbReview50886****

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)

Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labelling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000. Gating strategy was against CD45+, C11b+, GR1-cells.

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CD11c antibody [N418] (AB33483)

Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11c antibody [N418]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11c antibody [N418]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11c antibody [N418]

  • 660 APC

    APC Anti-CD11c antibody [N418]

  • 519 FITC

    FITC Anti-CD11c antibody [N418]

  • 578 PE

    PE Anti-CD11c antibody [N418]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11c antibody [N418]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11c antibody [N418]

  • Biotin

    Biotin Anti-CD11c antibody [N418]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11c antibody [N418]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11c antibody [N418]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

N418

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis (By similarity).
See full target information Itgax
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Publications (81)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed38127424

2023

Apoptosis recognition receptors regulate skin tissue repair in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Olivia Justynski,Kate Bridges,Will Krause,Maria Fernanda Forni,Quan M Phan,Teresa Sandoval-Schaefer,Kristyn Carter,Diane E King,Henry C Hsia,Michael I Gazes,Steven D Vyce,Ryan R Driskell,Kathryn Miller-Jensen,Valerie Horsley

JCI insight 8: PubMed36917181

2023

Thyroid hormone modulates hyperoxic neonatal lung injury and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Bianca M Vamesu,Teodora Nicola,Rui Li,Snehashis Hazra,Sadis Matalon,Naftali Kaminski,Namasivayam Ambalavanan,Jegen Kandasamy

Cell reports methods 3:100398 PubMed36936072

2023

Label-free cell segmentation of diverse lymphoid tissues in 2D and 3D.

Applications

Unspecified application

Species

Unspecified reactive species

John W Wills,Jack Robertson,Pani Tourlomousis,Clare M C Gillis,Claire M Barnes,Michelle Miniter,Rachel E Hewitt,Clare E Bryant,Huw D Summers,Jonathan J Powell,Paul Rees

Frontiers in immunology 13:931740 PubMed35865514

2022

Characterization of the Immunologic Phenotype of Dendritic Cells Infected With Herpes Simplex Virus 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Zhang,Xingli Xu,Suqin Duan,Yang Gao,Danjing Ma,Rong Yue,Fengyuan Zeng,Xueqi Li,Ziyan Meng,Xinghang Li,Zhenye Niu,Guorun Jiang,Li Yu,Yun Liao,Dandan Li,Lichun Wang,Heng Zhao,Ying Zhang,Qihan Li

Cell death discovery 8:94 PubMed35241649

2022

Concerted BAG3 and SIRPα blockade impairs pancreatic tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Margot De Marco,Vanessa Gauttier,Sabrina Pengam,Caroline Mary,Bianca Ranieri,Anna Basile,Michela Festa,Antonia Falco,Francesca Reppucci,Anna Lisa Cammarota,Fausto Acernese,Vincenzo De Laurenzi,Gianluca Sala,Sergio Brongo,Masayuki Miyasaka,Shabnam Shalapour,Bernard Vanhove,Nicolas Poirier,Roberta Iaccarino,Michael Karin,Maria Caterina Turco,Alessandra Rosati,Liberato Marzullo

Molecular medicine (Cambridge, Mass.) 28:4 PubMed35062863

2022

Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ signaling pathway in diabetic atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Liding Zhao,Ya Li,Tian Xu,Qingbo Lv,Xukun Bi,Xianglan Liu,Guosheng Fu,Yunzeng Zou,Junbo Ge,Zhaoyang Chen,Wenbin Zhang

Cancer immunology, immunotherapy : CII 71:2029-2040 PubMed35018481

2022

Vaccination against galectin-1 promotes cytotoxic T-cell infiltration in melanoma and reduces tumor burden.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Femel,Luuk van Hooren,Melanie Herre,Jessica Cedervall,Falk Saupe,Elisabeth J M Huijbers,Danielle R J Verboogen,Matthias Reichel,Victor L Thijssen,Arjan W Griffioen,Lars Hellman,Anna Dimberg,Anna-Karin Olsson

Frontiers in immunology 12:795554 PubMed34975901

2021

Spatial Point Pattern Analysis Identifies Mechanisms Shaping the Skin Parasite Landscape in Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes S P Doehl,Helen Ashwin,Najmeeyah Brown,Audrey Romano,Samuel Carmichael,Jon W Pitchford,Paul M Kaye

Cells 10: PubMed34571913

2021

Spore-Trained Dendritic Cells Enhance the Generation of Memory T Cells via ICAM1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Lin,Lulu Huang,Yuchen Li,Penghao Zhang,Qinghua Yu,Qian Yang

Annals of translational medicine 9:926 PubMed34350241

2021

Tregs-derived interleukin 35 attenuates endothelial proliferation through STAT1 in pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Naifu Wan,Wuwei Rong,Wentong Zhu,Daile Jia,Peiyuan Bai,Guizhu Liu,Qiangyou Wan,Ankang Lyu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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