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AB233173

Anti-CD11c antibody [N418] - BSA and Azide free

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Armenian hamster Monoclonal CD11c antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD11c, Integrin alpha-X, CD11 antigen-like family member C, Itgax

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Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (AB233173)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (AB233173)

Flow cytometry analysis of murine alveolar cells extracted using bronchoaveolar lavage in balb/c mice using 3mM PBS-EDTA, labeling CD11c with ab33483 at 1/2000, incubated for 20 mins at 20°C. Secondary used was ab175680 at 1/2000.

Gating strategy was against CD45+, C11b+, GR1-cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (AB233173)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD11c antibody [N418] - BSA and Azide free (AB233173)

Mouse splenic cells were incubated with anti-CD11c antibody ab33483 at 1/100 dilution for 30 min at 4°C. The secondary antibody used was goat anti-Armenian Hamster (Alexa Fluor® 488) preadsorbed at 1/100 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 PE-conjugated (ab22268) at 1/200 dilution.

Acquisition of >30,000 total events were collected. Gating strategy – events were collected with the forward and side light-scatter characteristics of viable lymphocytes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33483).

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11c antibody [N418]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11c antibody [N418]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11c antibody [N418]

  • 660 APC

    APC Anti-CD11c antibody [N418]

  • 519 FITC

    FITC Anti-CD11c antibody [N418]

  • 578 PE

    PE Anti-CD11c antibody [N418]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11c antibody [N418]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11c antibody [N418]

  • Biotin

    Biotin Anti-CD11c antibody [N418]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11c antibody [N418]

  • Carrier free

    Anti-CD11c antibody [N418] - Low endotoxin, Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11c antibody [N418]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

N418

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2000", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab233173 is the carrier-free version of ab33483.

Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.
Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis (By similarity).
See full target information Itgax
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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