AB13219

Anti-CD11+CD18 antibody [24]

Name: Anti-CD11+CD18 antibody [24] (ab13219) | Abcam
Brand: Abcam Limited
SKU: AB13219
Price: 475 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(28 Publications)

Mouse Monoclonal ITAL antibody. Suitable for ICC/IF, IP, Flow Cyt, WB, RIA and reacts with Human, Dog, Mouse, Rat samples. Cited in 28 publications. Immunogen corresponding to Cell preparation containing ITGB2 protein.

View Alternative Names

CD11a, CD11A, ITGAL, Integrin alpha-L, CD11 antigen-like family member A, Leukocyte adhesion glycoprotein LFA-1 alpha chain, Leukocyte function-associated molecule 1 alpha chain, LFA-1A, CD18, MFI7, ITGB2, Integrin beta-2, Complement receptor C3 subunit beta

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG1

Carrier free

Reacts with

Human, Dog, Rat, Mouse

Applications

IP, WB, Flow Cyt, ICC/IF, RIA

applications

Immunogen

Cell preparation containing ITGB2 protein. The exact immunogen used to generate this antibody is proprietary information.

P05107

Epitope

Recognises an epitope which is expressed by beta 2 (CD18) integrins in high affinity conformation.

Specificity

This antibody specifically recognises the human CD11+CD18 activation epitope.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" }, "Dog": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11+CD18 also known as integrin αLβ2 or LFA-1 is a heterodimeric protein complex with a molecular weight of approximately 180 kDa. It consists of two subunits CD11a (αL) and CD18 (β2) which are expressed on the surface of leukocytes such as neutrophils monocytes and lymphocytes. This complex plays an important role in cell-cell adhesion and interactions with the extracellular matrix. CD11+CD18 is an essential mediator of leukocyte adhesion and is involved in the immune response.

Biological function summary

CD11a/CD18 enables leukocyte adhesion to endothelial cells and guides their migration towards sites of inflammation. It functions as part of a larger integrin family responsible for various cellular processes. The protein complex binds to its ligand ICAM-1 (Intercellular Adhesion Molecule 1) initiating a cascade of signaling events that facilitate immune surveillance and cell junction formation. This adhesion complex is integral to the regulation of immune system functions.

Pathways

CD11a and CD18 participate in the leukocyte extravasation pathway which is important for trafficking immune cells to areas of injury or infection. The protein complex also interacts within the T-cell receptor signaling pathway assisting in T-cell activation and proliferation. It works alongside proteins such as ICAM-1 to maintain cellular communication and adhesion allowing for a coordinated immune response. The involvement of CD11+CD18 in these pathways highlights its role in mediating inflammatory responses.

CD11+CD18 is associated with inflammatory diseases like rheumatoid arthritis and leukocyte adhesion deficiency (LAD). In rheumatoid arthritis the overexpression and dysregulation of this integrin can contribute to the chronic inflammation characteristic of the disease. In LAD mutations affecting CD18 hinder normal leukocyte adhesion leading to recurrent infections. Aberrations in the expression or function of CD18 can affect other proteins in the immune system linking it to various immune-related disorders and immune system dysregulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed : 10846180, PubMed : 12526797, PubMed : 1676048, PubMed : 3086451). Integrin ITGAL/ITGB2 is a receptor for F11R (PubMed : 11812992, PubMed : 15528364). Integrin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL (PubMed : 29100055). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity (PubMed : 15356110). Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed : 11812992). Acts as a platform at the immunological synapse to translate TCR engagement and density of the ITGAL ligand ICAM1 into graded adhesion (PubMed : 38195629). Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis (By similarity). Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590). Integrins ITGAL : ITGB2 functions as a receptor of the neuron-specific adhesion molecule ICAM5 ensuring neuron cell-leukocyte adhesion (PubMed : 10741396). Integrin ITGAL/ITGB2 that functions as a signaling receptor of ICAM2, ensuring leukocyte cell-cell adhesion on resting cells (PubMed : 1676048).

See full target information ITGAL

Additional targets

ITGB2

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq8114 PubMed39792660

2025

Molecular dynamics at immune synapse lipid rafts influence the cytolytic behavior of CAR T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Z Gad,Jessica S Morris,Lea Godret-Miertschin,Melisa J Montalvo,Sybrina S Kerr,Harrison Berger,Jessica C H Lee,Amr M Saadeldin,Mohammad H Abu-Arja,Shuo Xu,Spyridoula Vasileiou,Rebecca M Brock,Kristen Fousek,Mohamed F Sheha,Madhuwanti Srinivasan,Yongshuai Li,Arash Saeedi,Kandice R Levental,Ann M Leen,Maksim Mamonkin,Alexandre Carisey,Navin Varadarajan,Meenakshi Hegde,Sujith K Joseph,Ilya Levental,Malini Mukherjee,Nabil Ahmed

Cardiovascular research 119:1811-1824 PubMed37279540

2023

Endothelial CCRL2 induced by disturbed flow promotes atherosclerosis via chemerin-dependent β2 integrin activation in monocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Chaojun Tang,Guona Chen,Fan Wu,Yiren Cao,Fei Yang,Tao You,Chu Liu,Menglu Li,Shuhong Hu,Lijie Ren,Qiongyu Lu,Wei Deng,Ying Xu,Guixue Wang,Hanjoong Jo,Yonghong Zhang,Yi Wu,Brian A Zabel,Li Zhu

Drug design, development and therapy 16:3677-3689 PubMed36277599

2022

The Mechanism of Alisol B23 Acetate Inhibiting Lung Cancer: Targeted Regulation of CD11b/CD18 to Influence Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yingna Chen,Jieya Lu,Zhihao Xie,Jialing Tang,Xuejiao Lian,Xiuwen Li

BMC biology 20:189 PubMed36002835

2022

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pascal Rossatti,Gregory M I Redpath,Luca Ziegler,Guerric P B Samson,Camille D Clamagirand,Daniel F Legler,Jérémie Rossy

Journal of nanobiotechnology 20:218 PubMed35525963

2022

Targeted neutrophil-mimetic liposomes promote cardiac repair by adsorbing proinflammatory cytokines and regulating the immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Yanan Song,Qiaozi Wang,Qiyu Li,Haipeng Tan,Jinfeng Gao,Ning Zhang,Xueyi Weng,Dili Sun,Wusiman Yakufu,Zhengmin Wang,Juying Qian,Zhiqing Pang,Zheyong Huang,Junbo Ge

PloS one 16:e0260835 PubMed34882718

2021

Key role of Extracellular RNA in hypoxic stress induced myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Saumya Bhagat,Indranil Biswas,Md Iqbal Alam,Madiha Khan,Gausal A Khan

Cancer immunology research 10:245-258 PubMed34819308

2021

C3aR Signaling Inhibits NK-cell Infiltration into the Tumor Microenvironment in Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Saravanan Nandagopal,Caiyun G Li,Yu Xu,Quaovi H Sodji,Edward E Graves,Amato J Giaccia

Theranostics 11:5248-5266 PubMed33859745

2021

Exosomal miR-125b-5p deriving from mesenchymal stem cells promotes tubular repair by suppression of p53 in ischemic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing-Yuan Cao,Bin Wang,Tao-Tao Tang,Yi Wen,Zuo-Lin Li,Song-Tao Feng,Min Wu,Dan Liu,Di Yin,Kun-Ling Ma,Ri-Ning Tang,Qiu-Li Wu,Hui-Yao Lan,Lin-Li Lv,Bi-Cheng Liu

Frontiers in immunology 11:594775 PubMed33424843

2020

Tumor Cell-Secreted ISG15 Promotes Tumor Cell Migration and Immune Suppression by Inducing the Macrophage M2-Like Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Ren-Hui Chen,Zhi-Wen Xiao,Xiao-Qing Yan,Ping Han,Fa-Ya Liang,Jing-Yi Wang,Shi-Tong Yu,Ting-Zhen Zhang,Si-Qi Chen,Qian Zhong,Xiao-Ming Huang

Journal of controlled release : official journal of the Controlled Release Society 330:101-110 PubMed33333118

2020

Genetically-engineered anti-PSMA exosome mimetics targeting advanced prostate cancer in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Maja Severic,Guanglong Ma,Sara G T Pereira,Amalia Ruiz,Calvin C L Cheung,Wafa T Al-Jamal

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CD11+CD18 antibody [24]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (28)

Product promise