AB95024

Anti-CD127 antibody

Name: Anti-CD127 antibody (ab95024) | Abcam
Brand: Abcam Limited
SKU: AB95024

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(4 Publications)

Rabbit Polyclonal CD127 antibody. Suitable for ELISA, Dot, WB, IHC-P and reacts with Synthetic peptide - Mouse, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Mouse Il7r.

View Alternative Names

CD127, Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, Il7r

2 Images

ELISA

Supplier Data

ELISA - Anti-CD127 antibody (AB95024)

ELISA results of ab95024 tested against BSA-conjugated peptide of immunizing peptide.

Each well was coated in duplicate with 0.1μg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log₁₀ of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC₅₀ is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody-Peroxidase and TMB ELISA peroxidase substrate.

Supplier Data

Dot Blot - Anti-CD127 antibody (AB95024)

Dot Blot - Anti-CD127 antibody (ab95024).

Antigen : Immunizing peptide, phosphorylated version of immunizing peptide and THRB1 and THRB1 pY171 as controls.

Load : 3.0, 0.3, 0.1, 0.05 μg as indicated.

Primary antibody : ab95024 at 1/400 for 45 min at 4°C.

Secondary antibody : Dylight™488 rabbit at 1/10,000 for 45 min at RT.

Block : 5% BLOTTO overnight at 4°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, Dot, ELISA, WB

applications

Immunogen

Synthetic Peptide within Mouse Il7r. The exact immunogen used to generate this antibody is proprietary information.

P16872

Specificity

This antibody is specific for mouse IL-7 receptor protein. A BLAST analysis was used to suggest cross-reactivity with IL-7 receptor protein from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 receptor protein from other sources has not been determined. Anti-IL-7 receptor alpha chain antibody is useful for researchers interested in Immunology Research.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using peptide coupled to agarose beads.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.

Biological function summary

CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.

Pathways

CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.

CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

See full target information Il7r

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & medicine 20: PubMed37381714

2023

Nuclear PLD1 combined with NPM1 induces gemcitabine resistance through tumorigenic IL7R in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Danqi Fu,Jingrui Yan,Zhaoyu Zhang,Yang Liu,Xiaoqing Ma,Jinsheng Ding,Shengyu Yang,Ran Zhao,Antao Chang,Chuntao Gao,Jing Liu,Tiansuo Zhao,Xiuchao Wang,Chongbiao Huang,Song Gao,Ying Ma,Bo Tang,Yukuan Feng,Hongwei Wang,Jihui Hao

Journal of cellular and molecular medicine 25:9939-9952 PubMed34581005

2021

Interleukin-7 aggravates myocardial ischaemia/reperfusion injury by regulating macrophage infiltration and polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Mengwen Yan,Yaliu Yang,Ying Zhou,Changan Yu,Rui Li,Wei Gong,Jingang Zheng

The ISME journal 14:2625-2645 PubMed32632263

2020

Coprophagy prevention alters microbiome, metabolism, neurochemistry, and cognitive behavior in a small mammal.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Bei Bo,Xue-Ying Zhang,Kevin D Kohl,Jing Wen,Shuang-Jie Tian,De-Hua Wang

International journal of clinical and experimental 11:1645-1652 PubMed31938264

2018

Elevated IL-7Rα is linked to recurrence and poorer survival of gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Jiang,Hao Yang,Hongzhou Wang,Mei Zheng,Baolong Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD127 antibody

ELISA - Anti-CD127 antibody (AB95024)

Dot Blot - Anti-CD127 antibody (AB95024)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise