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Rabbit Recombinant Monoclonal CD127 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL7R aa 1-250.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (AB314106), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (AB314106), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (AB314106), expandable thumbnail
  • Immunoprecipitation - Anti-CD127/IL-7R alpha antibody [BLR177J] (AB314106), expandable thumbnail
  • Western blot - Anti-CD127/IL-7R alpha antibody [BLR177J] (AB314106), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human IL7R aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16871

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CD127 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL7R aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human IL7R aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16871
Clone number

BLR177J

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.

Biological function summary

CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.

Pathways

CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.

Associated diseases and disorders

CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.

Product promise

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106)

    Detection of human CD127/IL-7R alpha in Hodgkin lymphoma by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106)

    Detection of human CD127/IL-7R alpha in spleen by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127/IL-7R alpha antibody [BLR177J] - BSA free (ab314106)

    Detection of human CD127/IL-7R alpha in metastatic lymph node by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

  • Immunoprecipitation - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106), expandable thumbnail

    Immunoprecipitation - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106)

    Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD127 recombinant monoclonal antibody [BLR177J] (A700-177) used for IP at 6 µl per reaction. CD127 was also immunoprecipitated by a second antibody against a different epitope of CD127 (BL-1919A-4E2). For blotting immunoprecipitated CD127, A700-177 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

  • Western blot - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106), expandable thumbnail

    Western blot - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106)

    Samples:Whole cell lysate (25 µg) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit anti-CD127 recombinant monoclonal antibody [BLR177J] (A700-177 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).

    All lanes: Western blot - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106) at 1/1000 dilution

    Lane 1: HeLa Whole cell lysate at 25 µg

    Lane 2: Daudi Whole cell lysate at 25 µg

    Lane 3: U2OS Whole cell lysate at 25 µg

    Lane 4: SUP-T1 Whole cell lysate at 25 µg

    Lane 5: Jurkat Whole cell lysate at 25 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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