Rabbit Recombinant Monoclonal CD127 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL7R aa 1-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
IHC-P | IP | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Interleukin-7 receptor subunit alpha, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, CDw127, IL7R
Rabbit Recombinant Monoclonal CD127 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL7R aa 1-250.
IgG
Rabbit
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Liquid
Monoclonal
BLR177J
Blue Ice
Up to 12 months
+4°C
+4°C
This supplementary information is collated from multiple sources and compiled automatically.
CD127 also known as IL-7R alpha or IL-7Rα is a protein found on the surface of cells involved in key immune functions. The CD127 protein has an approximate mass of 55-60 kDa. It is a component of the interleukin-7 receptor which also includes the common gamma chain. You can find CD127 expressed on various immune cells like T cells B cells and dendritic cells. As a marker CD127 is an important indicator of T cell maturity and function. Researchers often target CD127 in studies using antibodies in assay formats like ELISA to measure its expression level and function.
CD127 plays an important role in regulating lymphocyte development and survival. It is part of the IL-7R complex partnering with IL-7 for signal transduction that promotes cell proliferation and survival. This interaction is essential for the development and homeostasis of T cells and B cells. In the absence of proper CD127 signaling lymphocytes may suffer from poor survival impacting overall immune competence. The IL-7-CD127 interaction informs researchers about mechanisms of immune modulation and resilience.
CD127 participates in the JAK/STAT signaling pathway which is pivotal for transmitting information from extracellular signals to the cell nucleus influencing gene expression. This pathway plays a vital role in immune cell response. When IL-7 binds to the IL-7R complex on CD127 it initiates a cascade that involves proteins like JAK1 and STAT5. These proteins mediate responses necessary for cell proliferation and differentiation within immune system networks.
CD127 has implications in conditions like severe combined immunodeficiency (SCID) and autoimmune diseases. In SCID mutations or defects in CD127 cause impaired T cell development leading to compromised immune function. Additionally CD127 dysregulation can contribute to autoimmune disorders by affecting T regulatory cells creating an imbalance in immune response. Ocrevus a monoclonal antibody therapy used for multiple sclerosis indirectly relates to CD127 by targeting the B cells which rely on IL-7R signaling mechanisms for survival and function. Researchers continue to investigate CD127 to better understand its role in immune-related diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Detection of human CD127/IL-7R alpha in Hodgkin lymphoma by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human CD127/IL-7R alpha in spleen by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of human CD127/IL-7R alpha in metastatic lymph node by IHC. Antibody: Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody [BLR177J] (A700-177). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Samples: Whole cell lysate (0.5 mg per IP reaction; 20% of IP loaded) from Daudi cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD127 recombinant monoclonal antibody [BLR177J] (A700-177) used for IP at 6 µl per reaction. CD127 was also immunoprecipitated by a second antibody against a different epitope of CD127 (BL-1919A-4E2). For blotting immunoprecipitated CD127, A700-177 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.
Samples:Whole cell lysate (25 µg) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Antibody:Rabbit anti-CD127 recombinant monoclonal antibody [BLR177J] (A700-177 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
All lanes: Western blot - Anti-CD127/IL-7R alpha antibody [BLR177J] (ab314106) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 25 µg
Lane 2: Daudi Whole cell lysate at 25 µg
Lane 3: U2OS Whole cell lysate at 25 µg
Lane 4: SUP-T1 Whole cell lysate at 25 µg
Lane 5: Jurkat Whole cell lysate at 25 µg
All lanes: HRP-conjugated goat anti-rabbit IgG.
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